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Collagen I Rabbit pAb

Collagen I Rabbit pAb

     
  • IHC-P - Collagen I Rabbit pAb AP94681
    Tissue/cell: rat colon tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Collagen I Polyclonal Antibody, Unconjugated(AP94681) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
    detail
  • IHC-P - Collagen I Rabbit pAb AP94681
    Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Collagen I Polyclonal Antibody, Unconjugated(AP94681) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
    detail
  • WB - Collagen I Rabbit pAb AP94681
    Sample: Lane 1: Placenta (Mouse) Lysate at 40 ug Lane 2: U-2OS (Human) Cell Lysate at 30 ug Primary: Anti-Collagen I (AP94681) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 130,110 kD Observed band size: 130 kD
    detail
  • WB - Collagen I Rabbit pAb AP94681
    Sample: Lane 1: Placenta (Mouse) Lysate at 40 ug Lane 2: U-2OS (Human) Cell Lysate at 30 ug Primary: Anti-Collagen I (AP94681) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 130 kD Observed band size: 130 kD
    detail
  • WB - Collagen I Rabbit pAb AP94681
    Sample: Ovary (Mouse) Lysate at 40 ug NIH/3T3(Mouse) Cell Lysate at 40 ug Liver (Rat)Lysate at 40 ug 293T(huamn) Cell Lysate at 40 ug A431(huamn) Cell Lysate at 40 ug RAW264.7(Mouse) Cell Lysate at 40 ug Primary: Anti-Collagen I (AP94681) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 130 kD Observed band size: 110 kD
    detail
  •  - Collagen I Rabbit pAb AP94681
    Blank control:Hela. Primary Antibody (green line): Rabbit Anti-Collagen I antibody (AP94681) Dilution: 1ug/Test; Secondary Antibody : Goat anti-rabbit IgG-FITC Dilution: 0.5ug/Test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature.The secondary antibody used for 40 min at room temperature.Acquisition of 20,000 events was performed.
    detail
  • IHC-P - Collagen I Rabbit pAb AP94681
    Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Collagen I Polyclonal Antibody, Unconjugated (AP94681) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IHC-F, IF, E
Primary Accession P02452
Reactivity Human
Host Rabbit
Clonality Polyclonal
Calculated MW 138911 Da
Additional Information
Gene ID 1277
Other Names Collagen alpha-1(I) chain, Alpha-1 type I collagen, COL1A1
Dilution WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1ug/Test,ELISA=1:5000-10000
Format0.01M TBS(pH7.4), 0.09% (W/V) sodium azide and 50% Glyce
StorageStore at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ℃.
Protein Information
Name COL1A1
Function Type I collagen is a member of group I collagen (fibrillar forming collagen).
Cellular Location Secreted, extracellular space, extracellular matrix {ECO:0000255|PROSITE-ProRule:PRU00793}
Tissue Location Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite
Research Areas
Citations (0)
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Background

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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$ 385.00
Cat# AP94681
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