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LDHA Rabbit pAb

LDHA Rabbit pAb

     
  • WB - LDHA Rabbit pAb AP94839
    25 ug total protein per lane of various lysates (see on figure) probed with LDHA polyclonal antibody, unconjugated (AP94839) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
    detail
  • IHC-P - LDHA Rabbit pAb AP94839
    Paraformaldehyde-fixed, paraffin embedded Mouse Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with LDHA Polyclonal Antibody, Unconjugated (AP94839) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
    detail
  • IHC-P - LDHA Rabbit pAb AP94839
    Paraformaldehyde-fixed, paraffin embedded Human Skeletal muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with LDHA Polyclonal Antibody, Unconjugated (AP94839) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
    detail
  • IHC-P - LDHA Rabbit pAb AP94839
    Paraformaldehyde-fixed, paraffin embedded Mouse Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with LDHA Polyclonal Antibody, Unconjugated (AP94839) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
    detail
  • IHC-P - LDHA Rabbit pAb AP94839
    Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with LDHA Polyclonal Antibody, Unconjugated (AP94839) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
    detail
  • IHC-P - LDHA Rabbit pAb AP94839
    Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with LDHA Polyclonal Antibody, Unconjugated (AP94839) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IHC-F, IF
Primary Accession P00338
Reactivity Rat, Human, Mouse
Host Rabbit
Clonality Polyclonal
Calculated MW 37 KDa
Physical State Liquid
Immunogen KLH conjugated synthetic peptide derived from human LDHA
Isotype IgG
Purity affinity purified by Protein A
Buffer 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SUBCELLULAR LOCATION Cytoplasm.
SIMILARITY Belongs to the LDH/MDH superfamily. LDH family.
SUBUNIT Homotetramer.
Post-translational modifications ISGylated.
DISEASE Glycogen storage disease 11 (GSD11) [MIM:612933]: A metabolic disorder that results in exertional myoglobinuria, pain, cramps and easy fatigue. Note=The disease is caused by mutations affecting the gene represented in this entry.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Additional Information
Gene ID 3939
Other Names L-lactate dehydrogenase A chain, LDH-A, 1.1.1.27, Cell proliferation-inducing gene 19 protein, LDH muscle subunit, LDH-M, Renal carcinoma antigen NY-REN-59, LDHA (HGNC:6535)
Target/Specificity Metabolism of carbohydrates,Dehydrogenase Kits,NADP/NADPH,Carbohydrate metabolism,Energy Metabolism,Cancer,Energy Metabolism
Dilution WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:400-800,IF=1:10-50
Format0.01M TBS(pH7.4), 0.09% (W/V) sodium azide and 50% Glyce
StorageStore at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ℃.
Protein Information
Name LDHA (HGNC:6535)
Function Interconverts simultaneously and stereospecifically pyruvate and lactate with concomitant interconversion of NADH and NAD(+).
Cellular Location Cytoplasm.
Tissue Location Predominantly expressed in anaerobic tissues such as skeletal muscle and liver.
Research Areas
Citations (0)
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Background

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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Cat# AP94839
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