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RAB42 Rabbit pAb

RAB42 Rabbit pAb

     
  • FC - RAB42 Rabbit pAb AP94842
    Blank control:THP-1. Primary Antibody (green line): Rabbit Anti-RAB42 antibody (AP94842) Dilution: 2 µg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-FITC Dilution: 1 µg /test. Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
FC
Primary Accession Q8N4Z0
Reactivity Human
Host Rabbit
Clonality Polyclonal
Calculated MW 11 KDa
Physical State Liquid
Immunogen KLH conjugated synthetic peptide derived from human RAB42
Epitope Specificity 31-105/105
Isotype IgG
Purity affinity purified by Protein A
Buffer Preservative: 0.02% Proclin300, Constituents: 1% BSA, 0.01M PBS, pH7.4.
SUBCELLULAR LOCATION Membrane.
SIMILARITY Belongs to the small GTPase superfamily. Rab family.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Additional Information
Gene ID 115273
Other Names Ras-related protein Rab-42, 3.6.5.2, RAB42 (HGNC:28702)
Dilution Flow-Cyt=2ug/Test
Format0.01M TBS(pH7.4), 0.09% (W/V) sodium azide and 50% Glyce
StorageStore at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ℃.
Protein Information
Name RAB42 (HGNC:28702)
Function The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion (By similarity). The physiological function of RAB42 remains undefined (Probable).
Cellular Location Membrane {ECO:0000250|UniProtKB:P62820}; Lipid- anchor {ECO:0000250|UniProtKB:P62820}
Research Areas
Citations (0)
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Background

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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Cat# AP94842
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