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BLZF1 Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • IF - BLZF1 Antibody (Center) AP9600C
    Fluorescent image of A549 cell stained with BLZF1 Antibody (Center)(Cat#AP9600c).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with BLZF1 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃).BLZF1 immunoreactivity is localized to Nucleus significantly.
  • WB - BLZF1 Antibody (Center) AP9600C
    Western blot analysis of BLZF1 Antibody (Center) (Cat. #AP9600c) in HepG2 cell line lysates (35ug/lane). BLZF1 (arrow) was detected using the purified Pab.
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF, E
Primary Accession Q9H2G9
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 44910 Da
Antigen Region 156-184 aa
Additional Information
Gene ID 8548
Other Names Golgin-45, Basic leucine zipper nuclear factor 1, JEM-1, p45 basic leucine-zipper nuclear factor, BLZF1, JEM1
Target/Specificity This BLZF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 156-184 amino acids from the Central region of human BLZF1.
Dilution IF~~1:10~50
WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsBLZF1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name BLZF1
Synonyms JEM1
Function Required for normal Golgi structure and for protein transport from the endoplasmic reticulum (ER) through the Golgi apparatus to the cell surface.
Cellular Location Golgi apparatus membrane [Isoform 2]: Cytoplasm. Note=Not detected in the nucleus
Tissue Location Detected in adrenal gland (PubMed:9129147).
Research Areas
Citations (0)

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Background

Required for normal Golgi structure and for protein transport from the endoplasmic reticulum (ER) through the Golgi apparatus to the cell surface.

References

Ehret, G.B., et al. Eur. J. Hum. Genet. 17(12):1650-1657(2009)
Cheung, C.L., et al. Hum. Mol. Genet. 18(4):679-687(2009)
Reichman, T.W., et al. Mol. Cell. Biol. 22(1):343-356(2002)

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$ 162.50
$ 54.50
Cat# AP9600C
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