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>   home   >   Products   >   Primary Antibodies   >   Cell Biology   >   PKHH2 Antibody (C-term)   

PKHH2 Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - PKHH2 Antibody  (C-term) AP9773b
    Western blot analysis of PKHH2 Antibody (C-term) (Cat. #AP9773b) in Jurkat cell line lysates (35ug/lane). PKHH2 (arrow) was detected using the purified Pab.
  • IHC-P - PKHH2 Antibody  (C-term) AP9773b
    PKHH2 Antibody (C-term) (Cat. #AP9773b) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the APKHH2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
  • FC - PKHH2 Antibody  (C-term) AP9773b
    PKHH2 Antibody (C-term) (Cat. #AP9773b) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
FC, IHC-P, WB, E
Primary Accession Q8IVE3
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Antigen Region 1049-1079 aa
Additional Information
Other Names Pleckstrin homology domain-containing family H member 2, PLEKHH2, KIAA2028
Target/Specificity This PKHH2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1049-1079 amino acids from the C-terminal region of human PKHH2.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPKHH2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PLEKHH2
Synonyms KIAA2028
Function In the kidney glomerulus may play a role in linking podocyte foot processes to the glomerular basement membrane. May be involved in stabilization of F-actin by attenuating its depolymerization. Can recruit TGFB1I1 from focal adhesions to podocyte lamellipodia.
Cellular Location Cytoplasm. Cytoplasm, cytoskeleton. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, lamellipodium. Note=Localizes to foot process of podocytes. Localization to peripheral regions of lamellipodia seems to be dependent on PI3K
Tissue Location Kidney. Reduced expression in patients with focal segmental glomerulosclerosis. EMBL; AL832207; CAI46132.1; -; mRNA EMBL; AL833400; CAD38637.1; -; mRNA EMBL; BC063310; AAH63310.1; -; mRNA EMBL; AB095948; BAC23124.1; -; mRNA CCDS; CCDS1812.1; -. [Q8IVE3-1] RefSeq; NP_742066.2; NM_172069.3. [Q8IVE3-1] RefSeq; XP_016858842.1; XM_017003353.1. [Q8IVE3-2] BioGRID; 126228; 4 IntAct; Q8IVE3; 6 STRING; 9606.ENSP00000282406; - iPTMnet; Q8IVE3; - PhosphoSitePlus; Q8IVE3; - BioMuta; PLEKHH2; - DMDM; 158706383; - EPD; Q8IVE3; - jPOST; Q8IVE3; - MassIVE; Q8IVE3; - PaxDb; Q8IVE3; - PeptideAtlas; Q8IVE3; - PRIDE; Q8IVE3; - ProteomicsDB; 70683; -. [Q8IVE3-1] ProteomicsDB; 70684; -. [Q8IVE3-2] ProteomicsDB; 70685; -. [Q8IVE3-3] Antibodypedia; 29827; 82 antibodies DNASU; 130271; - Ensembl; ENST00000282406; ENSP00000282406; ENSG00000152527. [Q8IVE3-1] GeneID; 130271; - KEGG; hsa:130271; - UCSC; uc010yny.3; human. [Q8IVE3-1] CTD; 130271; - DisGeNET; 130271; - EuPathDB; HostDB:ENSG00000152527.13; - GeneCards; PLEKHH2; - HGNC; HGNC:30506; PLEKHH2 HPA; ENSG00000152527; Low tissue specificity MIM; 612723; gene neXtProt; NX_Q8IVE3; - OpenTargets; ENSG00000152527; - PharmGKB; PA134912826; - eggNOG; KOG0248; Eukaryota GeneTree; ENSGT00940000157675; - HOGENOM; CLU_001626_3_1_1; - InParanoid; Q8IVE3; - KO; K24020; - OMA; QTPCGSE; - OrthoDB; 75793at2759; - PhylomeDB; Q8IVE3; - TreeFam; TF312866; - PathwayCommons; Q8IVE3; - BioGRID-ORCS; 130271; 3 hits in 867 CRISPR screens ChiTaRS; PLEKHH2; human GenomeRNAi; 130271; - Pharos; Q8IVE3; Tdark PRO; PR:Q8IVE3; - Proteomes; UP000005640; Chromosome 2 RNAct; Q8IVE3; protein Bgee; ENSG00000152527; Expressed in lung and 193 other tissues ExpressionAtlas; Q8IVE3; baseline and differential Genevisible; Q8IVE3; HS GO; GO:0030864; C:cortical actin cytoskeleton; ISS:UniProtKB GO; GO:0005737; C:cytoplasm; IDA:UniProtKB GO; GO:0005829; C:cytosol; IDA:HPA GO; GO:0030027; C:lamellipodium; IDA:UniProtKB GO; GO:0016604; C:nuclear body; IDA:HPA GO; GO:0005654; C:nucleoplasm; IDA:HPA GO; GO:0005886; C:plasma membrane; IDA:UniProtKB GO; GO:0003779; F:actin binding; ISS:UniProtKB GO; GO:0042802; F:identical protein binding; IEA:Ensembl GO; GO:0030835; P:negative regulation of actin filament depolymerization; ISS:UniProtKB CDD; cd14473; FERM_B-lobe; 1 Gene3D; 1.20.80.10; -; 1 Gene3D; 1.25.40.530; -; 1 Gene3D; 2.30.29.30; -; 3 InterPro; IPR019749; Band_41_domain InterPro; IPR014352; FERM/acyl-CoA-bd_prot_sf InterPro; IPR035963; FERM_2 InterPro; IPR019748; FERM_central InterPro; IPR000299; FERM_domain InterPro; IPR000857; MyTH4_dom InterPro; IPR038185; MyTH4_dom_sf InterPro; IPR011993; PH-like_dom_sf InterPro; IPR001849; PH_domain Pfam; PF00373; FERM_M; 1 Pfam; PF00784; MyTH4; 1 Pfam; PF00169; PH; 1 SMART; SM00295; B41; 1 SMART; SM00139; MyTH4; 1 SMART; SM00233; PH; 2 SUPFAM; SSF47031; SSF47031; 1 PROSITE; PS50057; FERM_3; 1 PROSITE; PS51016; MYTH4; 1 PROSITE; PS50003; PH_DOMAIN; 2 1: Evidence at protein level; Alternative splicing; Cell membrane; Cell projection; Coiled coil; Cytoplasm; Cytoskeleton; Membrane; Polymorphism; Reference proteome; Repeat CHAIN 1..1493 /note="Pleckstrin homology domain-containing family H member 2" /id="PRO_0000307121" DOMAIN 703..797 /note="PH 1" /evidence="ECO:0000255|PROSITE-ProRule:PRU00145" DOMAIN 811..919 /note="PH 2" /evidence="ECO:0000255|PROSITE-ProRule:PRU00145" DOMAIN 955..1110 /note="MyTH4" /evidence="ECO:0000255|PROSITE-ProRule:PRU00359" DOMAIN 1121..1451 /note="FERM" /evidence="ECO:0000255|PROSITE-ProRule:PRU00084" COILED 20..175 /evidence="ECO:0000255" COMPBIAS 398..402 /note="Poly-Ser" COMPBIAS 613..666 /note="Ser-rich" COMPBIAS 693..696 /note="Poly-Ser" VAR_SEQ 1..563 /note="Missing (in isoform 2)" /evidence="ECO:0000303|PubMed:17974005" /id="VSP_028573" VAR_SEQ 768..796 /note="LTTEKHTYYLTADSPNILEEWIKVLQNVL -> VLNFFFFFFFFVSCQTQFS IIQPNGKEIG (in isoform 3)" /evidence="ECO:0000303|PubMed:15489334" /id="VSP_028574" VAR_SEQ 797..1493 /note="Missing (in isoform 3)" /evidence="ECO:0000303|PubMed:15489334" /id="VSP_028575" VARIANT 228 /note="M -> V (in dbSNP:rs10175843)" /id="VAR_055542" VARIANT 481 /note="P -> T (in dbSNP:rs17031297)" /id="VAR_035344" VARIANT 1069 /note="R -> K (in dbSNP:rs2278358)" /id="VAR_035345" VARIANT 1217 /note="N -> S (in dbSNP:rs17031368)" /id="VAR_035346" CONFLICT 451 /note="V -> A (in Ref. 1; CAI46132)" /evidence="ECO:0000305" CONFLICT 730 /note="G -> C (in Ref. 1; CAI46132)" /evidence="ECO:0000305" CONFLICT 858 /note="A -> V (in Ref. 1; CAI46132)" /evidence="ECO:0000305" SEQUENCE 1493 AA; 168229 MW; 5B7765F5FDDE7ED0 CRC64; MAELSEPEGP VDWKERCVAL ESQLMKFRVQ ASKIRELLAE KMQQLERQVI DAERQAEKAF QQVQVMEDKL KAANIQTSES ETRLYNKCQD LESLIQEKDD VIQNLELQLE EQKQIRIQEA KIIEEKAAKI KEWVTVKLNE LELENQNLRL INQNQTEEIR TMQSKLQEVQ GKKSSTVSTL KLSEGQRLSS LTFGCFLSRA RSPPQVVKSE EMSKISSKEP EFTEGKDMEE MEIPEKSVDN QVLENNRGQR TLHQTPCGSE QNRKTRTSFA TDGGISQNSG APVSDWSSDE EDGSKGRSKS RCTSTLSSHT SEEGVQCSRM GSEMYLTASD DSSSIFEEET FGIKRPEHKK LYSWQQEAQW KALNSPLGKG NSELSKKEQD SSSDELNKKF QSQRLDYSSS SSEANTPSPI LTPALMPKHP NSLSGKGTQL VPSSHLPPPK LRIPNVFSIS VALAKRHLSQ PQLSSDRMFG TNRNAISMIR PLRPQETDLD LVDGDSTEVL ENMDTSCDDG LFSYDSLDSP NSDDQEHCDS AKKVAYSKPP TPPLHRFPSW ESRIYAVAKS GIRMSEAFNM ESVNKNSAAT LSYTTSGLYT SLIYKNMTTP VYTTLKGKAT QISSSPFLDD SSGSEEEDSS RSSSRTSESD SRSRSGPGSP RAMKRGVSLS SVASESDYAI PPDAYSTDTE YSQPEQKLPK TCSSSSDNGK NEPLEKSGYL LKMSGKVKSW KRRWFVLKGG ELLYYKSPSD VIRKPQGHIE LSASCSILRG DNKQTVQLTT EKHTYYLTAD SPNILEEWIK VLQNVLRVQA ANPLSLQPEG KPTMKGLLTK VKHGYSKRVW CTLIGKTLYY FRSQEDKFPL GQIKLWEAKV EEVDRSCDSD EDYEASGRSL LSTHYTIVIH PKDQGPTYLL IGSKHEKDTW LYHLTVAAGS NNVNVGSEFE QLVCKLLNID GEPSSQIWRH PTLCHSKEGI ISPLTTLPSE ALQTEAIKLF KTCQLFINAA VDSPAIDYHI SLAQSALQIC LTHPELQNEI CCQLIKQTRR RQPQNQPGPL QGWQLLALCV GLFLPHHPFL WLLRLHLKRN ADSRTEFGKY AIYCQRCVER TQQNGDREAR PSRMEILSTL LRNPYHHSLP FSIPVHFMNG IYQVVGFDAS TTVEEFLNTL NQDTGMRKPA QSGFALFTDD PSGRDLEHCL QGNIKICDII SKWEQASKEQ QPGKCEGTRT VRLTYKNRLY FSVQARGETD REKLLLMYQT NDQIINGLFP LNKDLALEMA ALLSQVEIGD FERPFSTPAG HVTNQCKVNQ TLKQVIEKFY PKRYRDGCSE EQLRQLCQRL STRWMALRGH SAADCVRIYL TVARKWPFFG AKLFLAKPIT PSSLGSTFLW LAVHEDGLSL LEYNSMRLIV SYVYKSLMTF GGYQDDFMVV INNTHSKDKP TEKLLFAMAK PKILEITLLI ASYINNFHQQ KAAFHHLSAP ALLSAQTRGP QARMMGSQPL LSSSRPTKGP TLL
Research Areas
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References

Greene, C.N., et al. Hum. Genet. 124(3):255-262(2008)
Simpson, J.C., et al. EMBO Rep. 1(3):287-292(2000)

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Cat# AP9773b
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