|Application ||IHC-P, WB, E|
|Other Accession||Q29048, P50516, P31404|
|Predicted||Bovine, Mouse, Pig|
|Calculated MW||68304 Da|
|Antigen Region||441-468 aa|
|Other Names||V-type proton ATPase catalytic subunit A, V-ATPase subunit A, V-ATPase 69 kDa subunit, Vacuolar ATPase isoform VA68, Vacuolar proton pump subunit alpha, ATP6V1A, ATP6A1, ATP6V1A1, VPP2|
|Target/Specificity||This ATP6V1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 441-468 amino acids from the Central region of human ATP6V1A.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||ATP6V1A Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||ATP6A1, ATP6V1A1, VPP2|
|Function||Catalytic subunit of the peripheral V1 complex of vacuolar ATPase. V-ATPase vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. In aerobic conditions, involved in intracellular iron homeostasis, thus triggering the activity of Fe(2+) prolyl hydroxylase (PHD) enzymes, and leading to HIF1A hydroxylation and subsequent proteasomal degradation (PubMed:28296633). May play a role in neurite development and synaptic connectivity (PubMed:29668857).|
|Tissue Location||High expression in the skin.|
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Provided below are standard protocols that you may find useful for product applications.
This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is one of two V1 domain A subunit isoforms and is found in all tissues.
Martins-de-Souza, D., et al. J Psychiatr Res 43(11):978-986(2009)
Martins-de-Souza, D., et al. BMC Psychiatry 9, 17 (2009)
Lu, M., et al. J. Biol. Chem. 282(34):24495-24503(2007)
Ahmed, M., et al. J. Proteome Res. 4(3):931-940(2005)
Morel, N. Biol. Cell 95(7):453-457(2003)
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