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Hrk Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | O00198 |
| Other Accession | NP_003797, 4504493 |
| Reactivity | Human, Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Calculated MW | Predicted: 10 kDa Observed: 12 kDa |
| Application Notes | Hrk antibody can be used for the detection of Hrk by Western blot at 2.5 - 5 µg/mL. |
| Gene ID | 8739 |
|---|---|
| Other Names | Hrk Antibody: DP5, HARAKIRI, BID3, Activator of apoptosis harakiri, BH3-interacting domain-containing protein 3, harakiri, BCL2 interacting protein (contains only BH3 domain) |
| Target/Specificity | HRK; |
| Reconstitution & Storage | Hrk antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Precautions | Hrk Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | HRK |
|---|---|
| Synonyms | BID3 |
| Function | Promotes apoptosis. |
| Cellular Location | Membrane; Single-pass membrane protein. Mitochondrion |
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Provided below are standard protocols that you may find useful for product applications.
Background
Hrk Antibody: Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Hrk, a pro-apoptotic member of the Bcl-2 homology domain-3 (BH3)-only group of the Bcl-2 family of proteins, was also identified as novel protein induced during programmed neuronal death. It lacks significant homology to other Bcl-2 family members except for an 8-amino acid region that is similar to the BH3 motif of Bik. Hrk regulates apoptosis through interaction with the anti-apoptotic proteins Bcl-2 and Bcl-XL via this domain. It does not interact with the pro-apoptotic proteins Bax, Bak, or Bcl-XS. Hrk localizes to mitochondrial membranes in a pattern similar to that previously reported for Bcl-2 and Bcl-XL.
References
Lockshin RA, Osborne B, and Zakeri Z. Cell death in the third millennium. Cell Death Differ. 2000; 7:2-7.
Imaizumi K, Tsuda M, Imai Y, et al. Molecular cloning of a novel polypeptide, DP5, induced during programmed neuronal death. J. Biol. Chem. 1997; 272:18842-8.
Inohara N, Ding L, Chen S, et al. harakiri, a novel regulator of cell death, encodes a protein that activates apoptosis and interacts selectively with survival-promoting proteins Bcl-2 and Bcl-XL. EMBO J. 1997; 16:1686-94.
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