NKX2-2 Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application ![]()
| WB, IHC-P, IF, E |
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Primary Accession | O95096 |
Other Accession | NP_002500, 4505401 |
Reactivity | Human, Mouse, Rat |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | IgG |
Calculated MW | 30133 Da |
Application Notes | NKX2-2 antibody can be used for detection of NKX2-2 by Western blot at 1 µg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 µg/mL. For immunofluorescence start at 20 µg/mL. |
Gene ID | 4821 |
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Target/Specificity | NKX2-2; NKX2-2 antibody is predicted to not cross-react with other NK2 homeobox family members. |
Reconstitution & Storage | NKX2-2 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Precautions | NKX2-2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | NKX2-2 |
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Synonyms | NKX2.2, NKX2B |
Function | Transcriptional activator involved in the development of insulin-producting beta cells in the endocrine pancreas (By similarity). May also be involved in specifying diencephalic neuromeric boundaries, and in controlling the expression of genes that play a role in axonal guidance. Binds to elements within the NEUROD1 promoter (By similarity). |
Cellular Location | Nucleus {ECO:0000255|PROSITE-ProRule:PRU00108}. |

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Provided below are standard protocols that you may find useful for product applications.
Background
NKX2-2 Antibody: NKX2-2 (NK2 homeobox 2) is a member of a family of transcription factors that are involved in embryonic development and cell fate. NKX2-2 is crucial in the development of pancreatic islet cell differentiation and activates the ghrelin-promoter in these cells. NKX2-2 is expressed at the onset of pancreatic epithelium formation and becomes restricted to mature α-, β- and PP cells, and in NKX2-2-null mice embryos, all β-cells and most a-cells are replaced by ghrelin-positive cells. NKX2-2 is also thought to be involved in ventral neuronal patterning and floor plate development and commissural axon guidance.
References
Sussel L, Kalamaras J, Hartigan-O’Connor DJ, et al. Mice lacking the homeodomain transcription factor Nkx2.2 have diabetes due to arrested differentiation of pancreatic beta cells. Development 1998; 125:2213-21
Prado CL, Pugh-Bernard AE, Elghazi L, et al. Ghrelin cells replace insulin-producing beta cells in two mouse models of pancreas development. Proc. Natl. Acad. Sci. 2004; 101:2924-9.
Hill JT, Chao CS, Anderson KR, et al. Nkx2.2 activates the ghrelin promoter in pancreatic islet cells. Mol. Endocrinol. 2010; 381-90.
Briscoe J, Sussel L, Serup P, et al. Homeobox gene Nkx2.2 and specification of neuronal identity by graded Sonic hedgehog signaling. Nature 1999; 398:622-7.

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