Foundational characteristics of cancer include proliferation, angiogenesis, migration, evasion of apoptosis, and cellular immortality. Find key markers for these cellular processes and antibodies to detect them.
The SUMOplot™ Analysis Program predicts and scores sumoylation sites in your protein. SUMOylation is a post-translational modification involved in various cellular processes, such as nuclear-cytosolic transport, transcriptional regulation, apoptosis, protein stability, response to stress, and progression through the cell cycle.
The Autophagy Receptor Motif Plotter predicts and scores autophagy receptor binding sites in your protein. Identifying proteins connected to this pathway is critical to understanding the role of autophagy in physiological as well as pathological processes such as development, differentiation, neurodegenerative diseases, stress, infection, and cancer.
LMX1B Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC-P, IF, E |
|---|---|
| Primary Accession | O60663 |
| Other Accession | NP_001167617, 4010 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Calculated MW | 45 kDa |
| Application Notes | LMX1B antibody can be used for detection of LMX1B by Western blot at 1 - 2 μg/mL. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Gene ID | 4010 |
|---|---|
| Target/Specificity | Rabbit polyclonal LMX1B antibody was raised against a 17 amino acid peptide near the carboxy terminus of human LMX1B. The immunogen is located within amino acids 280 - 330 of LMX1B. |
| Reconstitution & Storage | LMX1B antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Precautions | LMX1B Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | LMX1B |
|---|---|
| Function | Transcription factor involved in the regulation of podocyte- expressed genes (PubMed:24042019, PubMed:28059119). Essential for the specification of dorsal limb fate at both the zeugopodal and autopodal levels. |
| Cellular Location | Nucleus {ECO:0000255|PROSITE-ProRule:PRU00108}. |
| Tissue Location | Expressed in most tissues. Highest levels in testis, thyroid, duodenum, skeletal muscle, and pancreatic islets |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
info@abcepta.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Background
LMX1B Antibody: The LIM homeobox transcription factor 1B (LMX1B) belongs to the LIM-homeodomain family. Members of this family are known to be important for pattern formation during development. LMX1B regulates mid-hindbrain patterning; LMX1B-null mice embryos have a severe reduction in the number of midbrain dopaminergic neurons compared to wild-type. While LMX1B appears to be important for both the development and the survival of dopamine neurons, the related LMX1A is crucial for the differentiation of these cells. However, LMX1A and LMX1B function cooperatively to regulated the proliferation, specification and differentiation of midbrain dopaminergic neuronal progenitors.
References
Hobert O and Westphal H. Functions of LIM-homeobox genes. Trends Genet. 2000; 16:75-83.
Nakatani T, Kumai M, Mizuhara E, et al. Lmx1a and Lmx1b cooperate with Foxa2 to coordinate the specification of dopaminergic neurons and control of floor plate cell differentiation in the developing mesencephalon. Dev. Biol. 2010; 339:101-13.
Guo C, Qiu HY, Huang Y, et al. Lmx1b is essential for FGF8 and Wnt1 expression in the isthmic organizer during tectum and cerebellum development in mice. Development 2007; 134:317-25.
Chizhikov VV and Millen KJ. Control of roof plate formation by LMX1A in the developing spinal cord. Development 2004; 131:2693-705.
If you have used an Abcepta product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at tech@abcepta.com.
Ordering Information
Other Products
Shipping Information
