XKR8 Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC-P, IF, E |
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Primary Accession | Q9H6D3 |
Other Accession | NP_060523, 24431977 |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | IgG |
Calculated MW | Predicted: 43 kDa Observed: 47 kDa |
Application Notes | XKR8 antibody can be used for detection of XKR8 by Western blot at 1 - 2 µg/ml. Antibody can also be used for immunohistochemistry starting at 5 µg/mL. For immunofluorescence start at 20 µg/mL. |
Gene ID | 55113 |
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Target/Specificity | XKR8; XKR8 antibody is human reactive. At least two isoforms are known to exist; this antibody will detect both isoforms. |
Reconstitution & Storage | XKR8 antibody can be stored at 4℃ for three months and -20℃, stable for up to one year. |
Precautions | XKR8 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | XKR8 {ECO:0000303|PubMed:23845944, ECO:0000312|HGNC:HGNC:25508} |
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Function | [XK-related protein 8, processed form]: Phospholipid scramblase that promotes phosphatidylserine exposure on apoptotic cell surface (PubMed:23845944, PubMed:25231987). Phosphatidylserine is a specific marker only present at the surface of apoptotic cells and acts as a specific signal for engulfment (PubMed:23845944). Required for the clearance of apoptotic cells, such as engulfment of apoptotic germ cells by Sertoli cells, clearance of senescent neutrophils or regulation of bipolar cell numbers in the retina (By similarity). Has no effect on calcium-induced exposure of phosphatidylserine (PubMed:23845944). Promotes myoblast differentiation and survival (PubMed:28881496). |
Cellular Location | Cell membrane; Multi-pass membrane protein. Cytoplasm, perinuclear region |
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Provided below are standard protocols that you may find useful for product applications.
Background
The XK Kell blood group complex subunit-related family member 8 (XKR8) is a member of a family of proteins that promote the phagocytosis of dying cells by altering the phospholipid distribution of the plasma membrane (1). XKR8 is thought to be a “scramblase”, an enzyme that eliminates the asymmetry of phospholipid distribution in the plasma membrane. In apoptotic cells, XKR8 promotes the cell-surface exposure of phosphatidylserine (PtdSer) which serves as a signal for phagocytosis (1,2).
References
Suzuki J, Denning DP, Imanishi E, et al. Xk-related protein 8 and CED-8 promote phosphatidylserine exposure in apoptotic cells. Science 2013; 341:403-6.
Wang X, Wu YC, Fadok VA, et al. Cell corpse engulfment mediated by C. elegans phosphatidylserine receptor through CED-5 and CED-12. Science 2003; 302:1563-6.
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