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COVID19
>   home   >   Products   >   Primary Antibodies   >   SARS-CoV-2 (COVID-19) ORF3a Antibody   

SARS-CoV-2 (COVID-19) ORF3a Antibody

Infectious Disease, COVID-19

     
  • IHC - SARS-CoV-2 (COVID-19) ORF3a Antibody ASC12217
    Figure 1 Immunohistochemistry Validation of SARS-CoV-2 (COVID-19) ORF3a in COVID-19 Patient Lung
    Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 (COVID-19) ORF3a antibody (9275, 0.2 μg/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 ORF3a protein was observed in macrophages of COVID-19 patient lung, but not in non-COVID-19 patient lung.
    detail
  • E - SARS-CoV-2 (COVID-19) ORF3a Antibody ASC12217
    Figure 2 ELISA Validation
    Antibodies: SARS-CoV-2 (COVID-19) ORF3a Antibody, 9275. A direct ELISA was performed using SARS-CoV-2 ORF3a immunogen peptide (9275P)) as coating antigen and the anti-SARS-CoV-2 (COVID-19) ORF3a antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.3 ng/mL to 1000 ng/mL
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC, E
Primary Accession P0DTC3
Other Accession P0DTC3
Host Rabbit
Clonality Polyclonal
Isotype IgG
Clone Names ORF3a
Calculated MW 31123 Da
Application Notes IHC: 0.2 μg/mL
Antibody validated: Immunohistochemistry in human samples. Antibody validated: SARS-CoV-2 (COVID-19) ORF3a antibody can detect 2 ng of free peptide at 1 μg/mL in ELISA. All other applications and species not yet tested.
Additional Information
Gene ID 43740569
Other Names ORF3a protein, Accessory protein 3a, Protein 3a, Protein U274, Protein X1, ORF3a
Reconstitution & Storage SARS-CoV-2 (COVID-19) ORF3a antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
PrecautionsSARS-CoV-2 (COVID-19) ORF3a Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name 3a
Function Plays a role in viral egress via lysosomal trafficking (PubMed:33157038, PubMed:33422265). Forms homotetrameric ion channels (viroporins) localized at endosomes and lysosomes, that may induce deacidification of lysosomes, allowing safe egress of virions via lysosomal trafficking (PubMed:33157038, PubMed:33422265, PubMed:34158638). Also blocks autolysosome formation by binding and sequestering the host component VPS39 for homotypic fusion and protein sorting (HOPS) on late endosomes (PubMed:33422265). This prevents fusion of autophagosomes with lysosomes, disrupting autophagy and facilitating virus egress (PubMed:33422265). Induces host RETREG1/FAM134B-dependent reticulophagy by interacting with host HMGB1 and enhancing the association between HMGB1 and host BECN1 (PubMed:35239449). This induces endoplasmic reticulum stress and inflammatory responses and facilitates viral infection (PubMed:35239449).
Cellular Location Virion {ECO:0000250|UniProtKB:P59632}. Host cell membrane {ECO:0000250|UniProtKB:P59632, ECO:0000269|PubMed:33060197, ECO:0000269|PubMed:34158638}; Multi-pass membrane protein {ECO:0000250|UniProtKB:P59632, ECO:0000269|PubMed:34158638}. Host endoplasmic reticulum membrane; Multi- pass membrane protein. Secreted {ECO:0000250|UniProtKB:P59632}. Host cytoplasm {ECO:0000250|UniProtKB:P59632, ECO:0000269|PubMed:33060197}. Host endosome Host lysosome. Note=The cell surface expressed protein can undergo endocytosis. The protein is secreted in association with membranous structures. {ECO:0000250|UniProtKB:P59632}
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Background

Coronavirus disease 2019 (COVID-19), formerly known as 2019-nCoV acute respiratory disease, is an infectious disease caused by SARS-CoV-2, a virus closely related to the SARS virus (1). The disease is the cause of the 2019–20 coronavirus outbreak (2). SARS-CoV-2 virus proteins include structural proteins, non-structural proteins and accessory factors. The structure of SARS-CoV-2 consists of the following: a spike protein (S), hemagglutinin-esterease dimer (HE), a membrane glycoprotein (M), an envelope protein (E) a nucleoclapid protein (N) and RNA. SARS-CoV-2 non-structural protein is ORF1ab that consists of 16 proteins (nsp1-nsp16), while accessory factors include ORF3a, ORF3b, ORF6, ORF7a, ORF7b, ORF8, ORF9b, ORF9c and ORF10. ORF3a forms homotetrameric potassium sensitive ion channels (viroporin) and may modulate virus release. It up-regulates expression of fibrinogen subunits FGA, FGB and FGG in host lung epithelial cells. It induces apoptosis in cell culture and downregulates the type 1 interferon receptor by inducing serine phosphorylation within the IFN alpha-receptor subunit 1 (IFNAR1) degradation motif and increasing IFNAR1 ubiquitination (3).

References

Gorbalenya. bioRxiv: 2020.;Hui et al. Int J Infect Dis. 2020;91:264-266.;Lu et al. PNAS USA. 2006; 103:12540-12545.

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$ 439.00
Cat# ASC12217
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