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TCF7 Antibody

Rabbit Anti-Human TCF7 Polyclonal

     
  •  - TCF7 Antibody ASM10573
    Immunohistochemistry analysis using Rabbit Anti-TCF7 Polyclonal Antibody (SPC-724). Tissue: Tonsil. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-TCF7 Polyclonal Antibody (SPC-724) at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 20X. | Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~41.6 kDa TCF7 protein using Rabbit Anti-TCF7 Polyclonal Antibody (SPC-724). Lane 1: Molecular Weight Ladder (MW). Lane 2: Cervical Cancer cell line (HeLa) lysate. Load: 10 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-TCF7 Polyclonal Antibody (SPC-724) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~41.6 kDa. Other Band(s): ~54 kDa isoform.
    detail
  •  - TCF7 Antibody ASM10573
    Immunohistochemistry analysis using Rabbit Anti-TCF7 Polyclonal Antibody (SPC-724). Tissue: Tonsil. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-TCF7 Polyclonal Antibody (SPC-724) at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 20X. | Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~41.6 kDa TCF7 protein using Rabbit Anti-TCF7 Polyclonal Antibody (SPC-724). Lane 1: Molecular Weight Ladder (MW). Lane 2: Cervical Cancer cell line (HeLa) lysate. Load: 10 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-TCF7 Polyclonal Antibody (SPC-724) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~41.6 kDa. Other Band(s): ~54 kDa isoform.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC
Primary Accession P36402
Other Accession NP_001128323.2
Host Rabbit
Clonality Polyclonal
Format TCF7
Target/Specificity TCF7
Other Names T-cell-specific transcription factor 1 Antibody, T-cell factor 1 Antibody, Transcription factor 7 Antibody, TCF7 Antibody, TCF-7 Antibody, TCF-1 Antibody, TCF1 Antibody
Clone Names TCF7
Immunogen Synthetic peptide from the C-terminal of Human TCF7 (284-384 aa), conjugated to Keyhole Limpet Haemocyanin (KLH).
Purification Peptide Affinity Purified
Storage -20ºC
Storage Buffer PBS pH 7.4, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
Shipping Temperature Blue Ice or 4ºC
Certificate of Analysis A 1:1000 dilution of SPC-724 was sufficient for detection of TCF7 in 15 µg of human HeLa cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
Cellular Localization Nucleus
Research Areas
Citations (0)
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$ 330.00
Cat# ASM10573
11 Formats Available
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