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EGFR Antibody

Rabbit Anti-Human EGFR Polyclonal

     
  •  - EGFR Antibody ASM10621
    Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~134.3 kDa EGFR protein using Rabbit Anti-EGFR Polyclonal Antibody (SPC-772). Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa. Load: 10 µg. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-EGFR Polyclonal Antibody (SPC-772) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~134.3 kDa. Other Band(s): 44 kDa and 69 kDa.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession P00533
Other Accession NP_005219.2
Host Rabbit
Clonality Polyclonal
Format EGFR
Target/Specificity EGFR
Other Names Cell growth inhibiting protein 40 Antibody, Avian erythroblastic leukemia viral (v erb b) oncogene homolog Antibody, Urogastrone Antibody, EGFR Antibody, Receptor tyrosine-protein kinase erbB-1 Antibody, v-erb-b Avian erythroblastic leukemia viral oncogen homolog Antibody, Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian) Antibody, Errp Antibody, PIG61 Antibody, erb-b2 receptor tyrosine kinase 1 Antibody, ERBB Antibody, mENA Antibody, Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) Antibody, ERBB1 Antibody, EGFR_HUMAN Antibody, SA7 Antibody, Proto-oncogene c-ErbB-1 Antibody, wa2 Antibody, Oncogen ERBB Antibody, Epidermal growth factor receptor Antibody, EGF R Antibody, Receptor tyrosine-protein kinase ErbB-1 Antibody, EC:2.7.10.1 Antibody, Cell proliferation inducing protein 61 Antibody, NISBD2 Antibody, Receptor tyrosine protein kinase ErbB 1 Antibody, HER1 Antibody, Wa5 Antibody, Species antigen 7 Antibody
Clone Names EGFR
Immunogen Synthetic peptide from the mid-protein of Human EGFR (aa. 200-300)
Purification Peptide Affinity Purified
Storage -20ºC
Storage Buffer PBS pH 7.4, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
Shipping Temperature Blue Ice or 4ºC
Cellular Localization Cell Membrane | Single-Pass Type I Membrane Protein | Endoplasmic Reticulum Membrane | Single-Pass Type I Membrane Protein | Golgi Apparatus Membrane | Single-Pass Type I Membrane Protein | Nucleus Membrane | Single-Pass Type I Membrane Protein | Endosome | Endosome Membrane | Nucleus
Research Areas
Citations (0)
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$ 330.00
Cat# ASM10621
11 Formats Available
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