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Anti-GOAT IgG (H&L) (Alkaline Phosphatase Conjugated) Secondary Antibody

Rabbit Polyclonal, Alkaline Phosphatase (Calf Intestine)

     
  • WB - Anti-GOAT IgG (H&L)  (Alkaline Phosphatase Conjugated) Secondary Antibody ASR2030
    Western Blot of Rabbit anti-Goat Antibody Alkaline Phosphatase Conjugated Lane 1: Goat IgG Load: 100 ng per lane Secondary antibody: Alkaline Phosphatase Conjugated Rabbit Anti-Goat Antibody at 1:1000 for 60 min at RT Block: MB-070 30 min RT Predicted/Observed size: 55 and 28 kDa, 55 and 28 kDa
    detail
  • DB - Anti-GOAT IgG (H&L)  (Alkaline Phosphatase Conjugated) Secondary Antibody ASR2030
    Dot Blot of Goat IgG (H&L) Secondary Antibody Alkaline Phosphatase Conjugated Antigen: Goat IgG Load: Lane 1 - 200ng Lane 2 - 66.7ng Lane 3 - 22.2ng Lane 4 - 7.4ng Lane 5 - 2.5ng Primary antibody: none Secondary antibody: Goat IgG (H&L) Secondary Antibody Alkaline Phosphatase Conjugated at 1:1,000 for 60 min at RT. Detected using Alkaline Phosphatase Substrate. Block: MB-070 60 min at RT.
    detail
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Product Information
Description Anti-GOAT IgG (H&L) (RABBIT) Antibody Alkaline Phosphatase Conjugated
Host Rabbit
Conjugate Alkaline Phosphatase (Calf Intestine)
Target Species Goat
Clonality Polyclonal
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, E, IC
Application Note ELISA 1:2,000-1:13,000
Western Blot 1:500-1:2,500
Immunochemistry 1:200-1:1,000
Physical State Liquid (sterile filtered)
Host Isotype IgG
Target Isotype IgG (H&L)
Buffer 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
Immunogen Goat IgG whole molecule
Stabilizer 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative 0.1% (w/v) Sodium Azide
Additional Information
Shipping Condition Wet Ice
Purity Anti-Goat Secondary Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Goat IgG and Goat Serum.
Storage Condition Store secondary antibody at 4° C before opening. DO NOT FREEZE. Anti-Goat secondary antibodies are stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.
Research Areas
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Background

Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

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$ 189.00
Cat# ASR2030
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