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Mouse IgG Fab fragment
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| Description | MOUSE IgG F(ab) fragment |
|---|---|
| Conjugate | Unconjugated |
| Physical State | Liquid (sterile filtered) |
| Host Isotype | IgG F(ab) |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Species of Origin | Mouse |
| Stabilizer | None |
| Preservative | 0.01% (w/v) Sodium Azide |
| Shipping Condition | Wet Ice |
|---|---|
| Purity | Mouse IgG F(ab) fragment was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Mouse IgG F(ab) fragment assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG, anti-Mouse IgG F(ab’)2 and anti-Mouse Serum. No reaction was observed against anti-Mouse IgG F(c) or anti- Papain. |
| Storage Condition | Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. |
| Precautions Note | This product is for research use only and is not intended for therapeutic or diagnostic applications. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The F(ab) fragment is the portion of the antibody that binds to the antigen target. The immunoglobulin F(ab) also possesses one constant and one variable region of both the heavy and light chain.Mouse IgG F(ab) Fragment is ideal for investigators in Immunology, Cancer, and Microbiology research.
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