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Anti-Human IgM (Fc5µ) Secondary Antibody
Mouse Monoclonal 22G1.H8 IgG2b kappa , Unconjugated
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- BACKGROUND
| Description | Anti-HUMAN IgM (Fc5 µ) (MOUSE) Antibody |
|---|---|
| Host | Mouse |
| Conjugate | Unconjugated |
| Target Species | Human |
| Reactivity | Human |
| Clonality | Monoclonal |
Application 
| WB, E |
| Application Note | ELISA 1:1000-1:10000 Western Blot 1:1000-1:2000 |
| Physical State | Liquid (sterile filtered) |
| Host Isotype | IgG2b |
| Target Isotype | IgM Fc5 µ |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Immunogen | Anti-Human IgM Fc5 µ was produced by repeated immunization with Human IgM Fc5 µ fragment in mice. |
| Stabilizer | None |
| Preservative | 0.01% (w/v) Sodium Azide |
| Shipping Condition | Dry Ice |
|---|---|
| Purity | Anti-Human IgM Fc5 µ Antibody was prepared from concentrated roller bottle supernatant by Protein A chromatography sepharose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum, Human IgM, Human Serum and Human Fc5 µ. No reaction was observed against Human IgG, Human IgA, Human Fc, Human F(ab) or Human F(ab)2. |
| Storage Condition | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Precautions Note | This product is for research use only and is not intended for therapeutic or diagnostic applications. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Human IgM Fc5 µ antibody generated in mice detects specifically human IgM. his anti-human IgM secondary antibody is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
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