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Anti-ACSS2 (RABBIT) Antibody
ACSS2 Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Host | Rabbit |
|---|---|
| Conjugate | Unconjugated |
| Target Species | Human |
| Reactivity | Human |
| Clonality | Polyclonal |
Application 
| WB, E, I, LCI |
| Application Note | Anti-ACSS2 Antibody has been tested in Western Blot and ELISA. Expect a band at approximately 76 kDa in western blot using appropriate lysates. Positive control whole cell lysates used Hep-G2 at 1 µg/mL for WB. Although not tested, this antibody is likely functional in immunohistochemistry, immunofluorescence, and immunoprecipitation. |
| Physical State | Liquid (sterile filtered) |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Immunogen | ACSS2 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with synthetic peptide near the N-term of human ACSS2. |
| Preservative | 0.01% (w/v) Sodium Azide |
| Gene ID | 55902 |
|---|---|
| Other Names | 55902 |
| Purity | This affinity purified antibody is directed against human ACSS2 protein. Anti-ACSS2 Antibody to was affinity purified from monospecific antiserum by immunoaffinity chromatography. Blast analysis of the sequence of the immunogen shows 100% identity with human and 80% identity to mouse. |
| Storage Condition | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Precautions Note | This product is for research use only and is not intended for therapeutic or diagnostic applications. |
| Name | ACSS2 |
|---|---|
| Synonyms | ACAS2 |
| Function | Catalyzes the synthesis of acetyl-CoA from short-chain fatty acids (PubMed:10843999, PubMed:28003429, PubMed:28552616). Acetate is the preferred substrate (PubMed:10843999, PubMed:28003429). Can also utilize propionate with a much lower affinity (By similarity). Nuclear ACSS2 promotes glucose deprivation-induced lysosomal biogenesis and autophagy, tumor cell survival and brain tumorigenesis (PubMed:28552616). Glucose deprivation results in AMPK-mediated phosphorylation of ACSS2 leading to its translocation to the nucleus where it binds to TFEB and locally produces acetyl-CoA for histone acetylation in the promoter regions of TFEB target genes thereby activating their transcription (PubMed:28552616). The regulation of genes associated with autophagy and lysosomal activity through ACSS2 is important for brain tumorigenesis and tumor survival (PubMed:28552616). Acts as a chromatin-bound transcriptional coactivator that up-regulates histone acetylation and expression of neuronal genes (By similarity). Can be recruited to the loci of memory-related neuronal genes to maintain a local acetyl-CoA pool, providing the substrate for histone acetylation and promoting the expression of specific genes, which is essential for maintaining long-term spatial memory (By similarity). |
| Cellular Location | Cytoplasm, cytosol. Cytoplasm {ECO:0000250|UniProtKB:Q9QXG4}. Nucleus Note=Glucose deprivation results in its AMPK-dependent phosphorylation and subsequent nuclear translocation (PubMed:28552616). Phosphorylation at Ser-659, leads to exposure of its nuclear localization signal which is required for its interaction with KPNA1 and subsequent translocation to the nucleus (PubMed:28552616). Found in the cytoplasm in undifferentiated neurons and upon differentiation, translocates to nucleus (By similarity). {ECO:0000250|UniProtKB:Q9QXG4, ECO:0000269|PubMed:28552616} |
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Provided below are standard protocols that you may find useful for product applications.
Background
This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI). ACSS2 (Acyl-CoA synthetase short-chain family member 2) is a protein coding gene encoding a cytosolic enzyme that catalyzes the activation of acetate for use in lipid synthesis and energy generation. This protein acts as a monomer and produces acetyl-CoA from acetate in a reaction that requires ATP. Expression of this gene is regulated by sterol regulatory element-binding proteins, transcription factors that activate genes required for the synthesis of cholesterol and unsaturated fatty acids. This protein is upregulated in many cancers and is important for pro-survival under hypoxic conditions. ACSS2 may be associated with disorders such as Cystoisosporiasis. Anti-ACSS2 Antibody is useful for researchers interested in parasites, intestines, Glucose Energy Metabolism, and AKT research.
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