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AATF Antibody (monoclonal) (M09)

Mouse monoclonal antibody raised against a partial recombinant AATF.

     
  • IF - AATF Antibody (monoclonal) (M09) AT1004a
    Immunofluorescence of monoclonal antibody to AATF on HeLa cell. [antibody concentration 10 ug/ml]
    detail
  • WB - AATF Antibody (monoclonal) (M09) AT1004a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.63 KDa) .
    detail
  • WB - AATF Antibody (monoclonal) (M09) AT1004a
    AATF monoclonal antibody (M09), clone 2H6 Western Blot analysis of AATF expression in HeLa ( (Cat # AT1004a )
    detail
  • E - AATF Antibody (monoclonal) (M09) AT1004a
    Detection limit for recombinant GST tagged AATF is 0.03 ng/ml as a capture antibody.
    detail
  • SPECIFICATION
  • CITATIONS
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  • BACKGROUND
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF, E
Primary Accession Q9NY61
Other Accession BC000591
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG1 Kappa
Clone Names 2H6
Calculated MW 63133 Da
Additional Information
Gene ID 26574
Other Names Protein AATF, Apoptosis-antagonizing transcription factor, Rb-binding protein Che-1, AATF, CHE1, DED
Target/Specificity AATF (AAH00591, 151 a.a. ~ 250 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsAATF Antibody (monoclonal) (M09) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
citation

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Background

The protein encoded by this gene was identified on the basis of its interaction with MAP3K12/DLK, a protein kinase known to be involved in the induction of cell apoptosis. This gene product contains a leucine zipper, which is a characteristic motif of transcription factors, and was shown to exhibit strong transactivation activity when fused to Gal4 DNA binding domain. Overexpression of this gene interfered with MAP3K12 induced apoptosis.

References

Che-1 promotes tumor cell survival by sustaining mutant p53 transcription and inhibiting DNA damage response activation. Bruno T, et al. Cancer Cell, 2010 Aug 9. PMID 20708154.Use of genome-wide expression data to mine the Gray Zone of GWA studies leads to novel candidate obesity genes. Naukkarinen J, et al. PLoS Genet, 2010 Jun 3. PMID 20532202.Mutation analysis of the AATF gene in breast cancer families. Haanp?? M, et al. BMC Cancer, 2009 Dec 21. PMID 20025740.Sequential use of transcriptional profiling, expression quantitative trait mapping, and gene association implicates MMP20 in human kidney aging. Wheeler HE, et al. PLoS Genet, 2009 Oct. PMID 19834535.Cellular AATF gene: armour against HIV-1. Kaul D. Indian J Biochem Biophys, 2007 Oct. PMID 18341201.

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$ 350.00
Cat# AT1004a
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Availability: 7-10 days
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