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ARHGEF1 Antibody (monoclonal) (M03)

Mouse monoclonal antibody raised against a partial recombinant ARHGEF1.

     
  • IF - ARHGEF1 Antibody (monoclonal) (M03) AT1185a
    Immunofluorescence of monoclonal antibody to ARHGEF1 on HeLa cell. [antibody concentration 10 ug/ml]
    detail
  • WB - ARHGEF1 Antibody (monoclonal) (M03) AT1185a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (36.41 KDa) .
    detail
  • WB - ARHGEF1 Antibody (monoclonal) (M03) AT1185a
    ARHGEF1 monoclonal antibody (M03), clone 4C4 Western Blot analysis of ARHGEF1 expression in K-562 ( (Cat # AT1185a )
    detail
  • E - ARHGEF1 Antibody (monoclonal) (M03) AT1185a
    Detection limit for recombinant GST tagged ARHGEF1 is approximately 0.3ng/ml as a capture antibody.
    detail
  • SPECIFICATION
  • CITATIONS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF, E
Primary Accession Q92888
Other Accession BC034013
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 4C4
Calculated MW 102435 Da
Additional Information
Gene ID 9138
Other Names Rho guanine nucleotide exchange factor 1, 115 kDa guanine nucleotide exchange factor, p115-RhoGEF, p115RhoGEF, Sub15, ARHGEF1
Target/Specificity ARHGEF1 (AAH34013.2, 830 a.a. ~ 927 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsARHGEF1 Antibody (monoclonal) (M03) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
citation

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Background

Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form complex with G proteins and stimulate Rho-dependent signals. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been defined.

References

1.Loss of lsc/p115-protein leads to neuronal hypoplasia in the esophagus and an achalasia-like phenotype in mice.Zizer E, Beilke S, Bauerle T, Schilling K, Mohnle U, Adler G, Fischer KD, Wagner M.Gastroenterology. 2010 Jun 20. [Epub ahead of print]

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$ 350.00
Cat# AT1185a
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Availability: 7-10 days
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