CFL1 Antibody (monoclonal) (M04)
Mouse monoclonal antibody raised against a full length recombinant CFL1.
|Application ||WB, IHC, IF|
|Calculated MW||18502 Da|
|Other Names||Cofilin-1, 18 kDa phosphoprotein, p18, Cofilin, non-muscle isoform, CFL1, CFL|
|Target/Specificity||CFL1 (AAH11005, 1 a.a. ~ 166 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.|
|Format||Clear, colorless solution in phosphate buffered saline, pH 7.2 .|
|Storage||Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.|
|Precautions||CFL1 Antibody (monoclonal) (M04) is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.
1.Determination of urine cofilin-1 level in acute kidney injury using a high-throughput localized surface plasmon-coupled fluorescence biosensor.Chang YF, Chao CH, Lin LY, Tsai CH, Chou C, Lee YJJ Biomed Opt. 2014 Jan;19(1):11004. doi: 10.1117/1.JBO.19.1.011004.2.Identification of phosphorylated serine-15 and -82 residues of HSPB1 in 5-fluorouracil- resistant colorectal cancer cells by proteomics.Sakai A, Otani M, Miyamoto A, Yoshida H, Furuya E, Tanigawa N.Journal of Proteomics (2011), doi: 10.1016/j.jprot.2011.09.0233.Overexpression of cofilin 1 can predict progression-free survival in patients with epithelial ovarian cancer receiving standard therapy.Nishimura S, Tsuda H, Kataoka F, Arao T, Nomura H, Chiyoda T, Susumu N, Nishio K, Aoki D.Hum Pathol. 2011 Jan 13. [Epub ahead of print]
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