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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   ENO1 Antibody (monoclonal) (M01)   

ENO1 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a full length recombinant ENO1.

  • IF - ENO1 Antibody (monoclonal) (M01) AT1908a
    Immunofluorescence of monoclonal antibody to ENO1 on HeLa cell. [antibody concentration 10 ug/ml]
  • IP - ENO1 Antibody (monoclonal) (M01) AT1908a
    Immunoprecipitation of ENO1 transfected lysate using anti-ENO1 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with ENO1 MaxPab rabbit polyclonal antibody.
  • WB - ENO1 Antibody (monoclonal) (M01) AT1908a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (73.48 KDa) .
  • WB - ENO1 Antibody (monoclonal) (M01) AT1908a
    ENO1 monoclonal antibody (M01), clone 8G8. Western Blot analysis of ENO1 expression in human colon.
  • WB - ENO1 Antibody (monoclonal) (M01) AT1908a
    ENO1 monoclonal antibody (M01), clone 8G8 Western Blot analysis of ENO1 expression in MCF-7 ( (Cat # AT1908a )
  • WB - ENO1 Antibody (monoclonal) (M01) AT1908a
    Western Blot analysis of ENO1 expression in transfected 293T cell line by ENO1 monoclonal antibody (M01), clone 8G8.

    Lane 1: ENO1 transfected lysate(47 KDa).
    Lane 2: Non-transfected lysate.
  • IHC - ENO1 Antibody (monoclonal) (M01) AT1908a
    Immunoperoxidase of monoclonal antibody to ENO1 on formalin-fixed paraffin-embedded human lymphoma tissue. [antibody concentration 1 ug/ml]
  • E - ENO1 Antibody (monoclonal) (M01) AT1908a
    Detection limit for recombinant GST tagged ENO1 is approximately 0.3ng/ml as a capture antibody.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P06733
Other Accession BC022545
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1 kappa
Clone Names 8G8
Calculated MW 47169 Da
Additional Information
Gene ID 2023
Other Names Alpha-enolase, 2-phospho-D-glycerate hydro-lyase, C-myc promoter-binding protein, Enolase 1, MBP-1, MPB-1, Non-neural enolase, NNE, Phosphopyruvate hydratase, Plasminogen-binding protein, ENO1, ENO1L1, MBPB1, MPB1
Target/Specificity ENO1 (AAH22545, 1 a.a. ~ 434 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsENO1 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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This gene encodes one of three enolase isoenzymes found in mammals; it encodes alpha-enolase, a homodimeric soluble enzyme, and also encodes a shorter monomeric structural lens protein, tau-crystallin. The two proteins are made from the same message. The full length protein, the isoenzyme, is found in the cytoplasm. The shorter protein is produced from an alternative translation start, is localized to the nucleus, and has been found to bind to an element in the c-myc promoter. A pseudogene has been identified that is located on the other arm of the same chromosome.


1.The interaction with caveolae-associated proteins regulates enolase-1 subcellular localization.Zakrzewicz D, Didiasova M, Zakrzewicz A, Hocke AC, Uhle F, Markart P, Preissner KT, Wygrecka MBiochem J. 2014 Jun 1;460(2):295-307. doi: 10.1042/BJ20130945.2.Overexpression of alpha-enolase correlates with poor survival in canine mammary carcinoma.Chu PY, Hsu NC, Liao AT, Shih NY, Hou MF, Liu CH.BMC Vet Res. 2011 Oct 21;7:62.3.Systematic proteomic analysis of human hepotacellular carcinoma cells reveals molecular pathways and networks involved in metastasis.Yu Y, Shen H, Yu H, Zhong F, Zhang Y, Zhang C, Zhao J, Li H, Chen J, Liu Y, Yang P.Mol Biosyst. 2011 Jun;7(6):1908-16. Epub 2011 Apr 6.4.Diagnostic Detection of Human Lung Cancer-Associated Antigen Using a Gold Nanoparticle-Based Electrochemical Immunosensor.Ho JA, Chang HC, Shih NY, Wu LC, Chang YF, Chen CC, Chou C.Anal Chem. 2010 Jun 17. [Epub ahead of print]5.Comparative proteomic analysis of Barrett's metaplasia and esophageal adenocarcinomas using 2-D liquid mass mapping.Zhao J, Chang AC, Li C, Shedden KA, Thomas DG, Misek DE, Manoharan AP, Giordano TJ, Beer DG, Lubman DM.Mol Cell Proteomics. 2007 Jun;6(6):987-99. Epub 2006 Jul 8.

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$ 350.00
Cat# AT1908a
Availability: 7-10 days
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