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ENO1 Antibody (monoclonal) (M01)
Mouse monoclonal antibody raised against a full length recombinant ENO1.
- SPECIFICATION
- CITATIONS: 1
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC, IF, IP, E |
|---|---|
| Primary Accession | P06733 |
| Other Accession | BC022545 |
| Reactivity | Human |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 kappa |
| Clone Names | 8G8 |
| Calculated MW | 47169 Da |
| Gene ID | 2023 |
|---|---|
| Other Names | Alpha-enolase, 2-phospho-D-glycerate hydro-lyase, C-myc promoter-binding protein, Enolase 1, MBP-1, MPB-1, Non-neural enolase, NNE, Phosphopyruvate hydratase, Plasminogen-binding protein, ENO1, ENO1L1, MBPB1, MPB1 |
| Target/Specificity | ENO1 (AAH22545, 1 a.a. ~ 434 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
| Dilution | WB~~1:500~1000 |
| Format | Clear, colorless solution in phosphate buffered saline, pH 7.2 . |
| Storage | Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing. |
| Precautions | ENO1 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures. |
Provided below are standard protocols that you may find useful for product applications.
Background
This gene encodes one of three enolase isoenzymes found in mammals; it encodes alpha-enolase, a homodimeric soluble enzyme, and also encodes a shorter monomeric structural lens protein, tau-crystallin. The two proteins are made from the same message. The full length protein, the isoenzyme, is found in the cytoplasm. The shorter protein is produced from an alternative translation start, is localized to the nucleus, and has been found to bind to an element in the c-myc promoter. A pseudogene has been identified that is located on the other arm of the same chromosome.
References
1.The interaction with caveolae-associated proteins regulates enolase-1 subcellular localization.Zakrzewicz D, Didiasova M, Zakrzewicz A, Hocke AC, Uhle F, Markart P, Preissner KT, Wygrecka MBiochem J. 2014 Jun 1;460(2):295-307. doi: 10.1042/BJ20130945.2.Overexpression of alpha-enolase correlates with poor survival in canine mammary carcinoma.Chu PY, Hsu NC, Liao AT, Shih NY, Hou MF, Liu CH.BMC Vet Res. 2011 Oct 21;7:62.3.Systematic proteomic analysis of human hepotacellular carcinoma cells reveals molecular pathways and networks involved in metastasis.Yu Y, Shen H, Yu H, Zhong F, Zhang Y, Zhang C, Zhao J, Li H, Chen J, Liu Y, Yang P.Mol Biosyst. 2011 Jun;7(6):1908-16. Epub 2011 Apr 6.4.Diagnostic Detection of Human Lung Cancer-Associated Antigen Using a Gold Nanoparticle-Based Electrochemical Immunosensor.Ho JA, Chang HC, Shih NY, Wu LC, Chang YF, Chen CC, Chou C.Anal Chem. 2010 Jun 17. [Epub ahead of print]5.Comparative proteomic analysis of Barrett's metaplasia and esophageal adenocarcinomas using 2-D liquid mass mapping.Zhao J, Chang AC, Li C, Shedden KA, Thomas DG, Misek DE, Manoharan AP, Giordano TJ, Beer DG, Lubman DM.Mol Cell Proteomics. 2007 Jun;6(6):987-99. Epub 2006 Jul 8.
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