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GCLC Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant GCLC.

     
  • IF - GCLC Antibody (monoclonal) (M01) AT2176a
    Immunofluorescence of monoclonal antibody to GCLC on HeLa cell. [antibody concentration 10 ug/ml]
    detail
  • WB - GCLC Antibody (monoclonal) (M01) AT2176a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (37.84 KDa) .
    detail
  • WB - GCLC Antibody (monoclonal) (M01) AT2176a
    GCLC monoclonal antibody (M01), clone 3H1. Western Blot analysis of GCLC expression in PC-12.
    detail
  • WB - GCLC Antibody (monoclonal) (M01) AT2176a
    GCLC monoclonal antibody (M01), clone 3H1 Western Blot analysis of GCLC expression in A-431 ( (Cat # AT2176a )
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  • WB - GCLC Antibody (monoclonal) (M01) AT2176a
    GCLC monoclonal antibody (M01), clone 3H1. Western Blot analysis of GCLC expression in NIH/3T3.
    detail
  • WB - GCLC Antibody (monoclonal) (M01) AT2176a
    Western Blot analysis of GCLC expression in transfected 293T cell line by GCLC monoclonal antibody (M01), clone 3H1.

    Lane 1: GCLC transfected lysate(72.8 KDa).
    Lane 2: Non-transfected lysate.
    detail
  • E - GCLC Antibody (monoclonal) (M01) AT2176a
    Detection limit for recombinant GST tagged GCLC is approximately 0.03ng/ml as a capture antibody.
    detail
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF
Primary Accession P48506
Other Accession NM_001498
Reactivity Human, Mouse, Rat
Host mouse
Clonality Monoclonal
Isotype IgG1 Kappa
Clone Names 3H1
Calculated MW 72766 Da
Additional Information
Gene ID 2729
Other Names Glutamate--cysteine ligase catalytic subunit, GCS heavy chain, Gamma-ECS, Gamma-glutamylcysteine synthetase, GCLC, GLCL, GLCLC
Target/Specificity GCLC (NP_001489, 528 a.a. ~ 637 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsGCLC Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
citation

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Background

Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. The gene encoding the catalytic subunit encodes a protein of 367 amino acids with a calculated molecular weight of 72.773 kDa and maps to chromosome 6. The regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Deficiency of gamma-glutamylcysteine synthetase in human is associated with enzymopathic hemolytic anemia.

References

1.Prominent steatosis with hypermetabolism of the cell line permissive for years of infection with hepatitis C virus.Sugiyama K, Ebinuma H, Nakamoto N, Sakasegawa N, Murakami Y, Chu PS, Usui S, Ishibashi Y, Wakayama Y, Taniki N, Murata H, Saito Y, Fukasawa M, Saito K, Yamagishi Y, Wakita T, Takaku H, Hibi T, Saito H, Kanai TPLoS One. 2014 Apr 9;9(4):e94460. doi: 10.1371/journal.pone.0094460. eCollection 2014.2.The LEGSKO Mouse: A Mouse Model of Age-Related Nuclear Cataract Based on Genetic Suppression of Lens Glutathione Synthesis.Fan X, Liu X, Hao S, Wang B, Robinson ML, Monnier VM.PLoS One. 2012;7(11):e50832. doi: 10.1371/journal.pone.0050832. Epub 2012 Nov 30.3.Differential activation of the inflammasome in THP-1 cells exposed to chrysotile asbestos and Libby"six-mix"amphiboles and subsequent activation of BEAS-2B cells.Li M, Gunter ME, Fukagawa NK.Cytokine. 2012 Sep 24. pii: S1043-4666(12)00666-7. doi: 10.1016/j.cyto.2012.08.025.4.Protection against 2-chloroethyl ethyl sulfide (CEES) - Induced cytotoxicity in human keratinocytes by an inducer of the glutathione detoxification pathway.Abel EL, Bubel JD, Simper MS, Powell L, McClellan SA, Andreeff M, Macleod MC, Digiovanni J.Toxicol Appl Pharmacol. 2011 Jun 23. [Epub ahead of print]5.Diversity in Antioxidant Response Enzymes in Progressive Stages of Human Nonalcoholic Fatty Liver Disease.Hardwick RN, Fisher CD, Canet MJ, Lake AD, Cherrington NJ.Drug Metab Dispos. 2010 Dec;38(12):2293-301. Epub 2010 Aug 30.

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$ 350.00
Cat# AT2176a
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