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IL27 Antibody (monoclonal) (M01)

Mouse monoclonal antibody raised against a partial recombinant IL27.

     
  • IP - IL27 Antibody (monoclonal) (M01) AT2518a
    Immunoprecipitation of IL27 transfected lysate using anti-IL27 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with IL27 MaxPab rabbit polyclonal antibody.
    detail
  • WB - IL27 Antibody (monoclonal) (M01) AT2518a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (33.11 KDa) .
    detail
  • WB - IL27 Antibody (monoclonal) (M01) AT2518a
    IL27 monoclonal antibody (M01), clone 3F12 Western Blot analysis of IL27 expression in HeLa ( (Cat # AT2518a )
    detail
  • WB - IL27 Antibody (monoclonal) (M01) AT2518a
    Western Blot analysis of IL27 expression in transfected 293T cell line by IL27 monoclonal antibody (M01), clone 3F12.

    Lane 1: IL27 transfected lysate(27.5 KDa).
    Lane 2: Non-transfected lysate.
    detail
  • SPECIFICATION
  • CITATIONS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IP
Primary Accession Q8NEV9
Other Accession NM_145659
Reactivity Human
Host mouse
Clonality Monoclonal
Isotype IgG1 Kappa
Clone Names 3F12
Calculated MW 27493 Da
Additional Information
Gene ID 246778
Other Names Interleukin-27 subunit alpha, IL-27 subunit alpha, IL-27-A, IL27-A, Interleukin-30, p28, IL27, IL27A, IL30
Target/Specificity IL27 (NP_663634, 177 a.a. ~ 243 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsIL27 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
citation

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Background

The protein encoded by this gene is one of the subunits of a heterodimeric cytokine complex. This protein is related to interleukin 12A (IL12A). It interacts with Epstein-Barr virus induced gene 3 (EBI3), a protein similar to interleukin 12B (IL12B), and forms a complex that has been shown to drive rapid expansion of naive but not memory CD4(+) T cells. The complex is also found to synergize strongly with interleukin 12 to trigger interferon gamma (IFNG) production of naive CD4(+) T cells. The biological effects of this cytokine are mediated by the class I cytokine receptor (WSX1/TCRR).

References

Interleukin-9 polymorphism in infants with respiratory syncytial virus infection: an opposite effect in boys and girls. Schuurhof A, et al. Pediatr Pulmonol, 2010 Jun. PMID 20503287.A pivotal role for interleukin-27 in CD8+ T cell functions and generation of cytotoxic T lymphocytes. Morishima N, et al. J Biomed Biotechnol, 2010. PMID 20454646.Activation of IL-27 p28 gene transcription by interferon regulatory factor 8 in cooperation with interferon regulatory factor 1. Zhang J, et al. J Biol Chem, 2010 Jul 9. PMID 20435892.Cytokines involved in interferon-gamma production by human macrophages. Robinson CM, et al. J Innate Immun, 2009 Dec. PMID 20375623.Activation of human bronchial epithelial cells by inflammatory cytokines IL-27 and TNF-alpha: implications for immunopathophysiology of airway inflammation. Cao J, et al. J Cell Physiol, 2010 Jun. PMID 20301193.

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$ 350.00
Cat# AT2518a
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Availability: 7-10 days
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