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MRPL12 Antibody (monoclonal) (M01)
Mouse monoclonal antibody raised against a full length recombinant MRPL12.
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC, IF, E |
|---|---|
| Primary Accession | P52815 |
| Other Accession | BC002344 |
| Reactivity | Human |
| Host | mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 kappa |
| Clone Names | 3B12-1A3 |
| Calculated MW | 21348 Da |
| Gene ID | 6182 |
|---|---|
| Other Names | 39S ribosomal protein L12, mitochondrial, L12mt, MRP-L12, 5c5-2, MRPL12, RPML12 |
| Target/Specificity | MRPL12 (AAH02344, 1 a.a. ~ 198 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
| Dilution | WB~~1:500~1000 IHC~~1:100~500 IF~~1:50~200 E~~N/A |
| Format | Clear, colorless solution in phosphate buffered saline, pH 7.2 . |
| Storage | Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing. |
| Precautions | MRPL12 Antibody (monoclonal) (M01) is for research use only and not for use in diagnostic or therapeutic procedures. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein which forms homodimers. In prokaryotic ribosomes, two L7/L12 dimers and one L10 protein form the L8 protein complex.
References
1.A bifunctional protein regulates mitochondrial protein synthesis.Richman TR, Davies SM, Shearwood AM, Ermer JA, Scott LH, Hibbs ME, Rackham O, Filipovska ANucleic Acids Res. 2014 Mar 5.2.Oxygen Consumption Can Regulate the Growth of Tumors, a New Perspective on the Warburg Effect.Chen Y, Cairns R, Papandreou I, Koong A, Denko NC.PLoS One. 2009 Sep 15;4(9):e7033.3.Human mitochondrial ribosomal protein MRPL12 interacts directly with mitochondrial RNA polymerase to modulate mitochondrial gene expression.Wang Z, Cotney J, Shadel GS.J Biol Chem. 2007 Apr 27;282(17):12610-8. Epub 2007 Mar 2.
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