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NEK2 Antibody (monoclonal) (M11)

Mouse monoclonal antibody raised against a partial recombinant NEK2.

  • IF - NEK2 Antibody (monoclonal) (M11) AT3016a
    Immunofluorescence of monoclonal antibody to NEK2 on HeLa cell. [antibody concentration 25 ug/ml]
  • WB - NEK2 Antibody (monoclonal) (M11) AT3016a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (38.28 KDa) .
  • WB - NEK2 Antibody (monoclonal) (M11) AT3016a
    NEK2 monoclonal antibody (M11), clone 3B7. Western Blot analysis of NEK2 expression in PC-12 ( (Cat # AT3016a )
  • WB - NEK2 Antibody (monoclonal) (M11) AT3016a
    NEK2 monoclonal antibody (M11), clone 3B7. Western Blot analysis of NEK2 expression in Raw 264.7 ( (Cat # AT3016a )
  • WB - NEK2 Antibody (monoclonal) (M11) AT3016a
    NEK2 monoclonal antibody (M11), clone 3B7 Western Blot analysis of NEK2 expression in MCF-7 ( (Cat # AT3016a )
  • WB - NEK2 Antibody (monoclonal) (M11) AT3016a
    NEK2 monoclonal antibody (M11), clone 3B7. Western Blot analysis of NEK2 expression in NIH/3T3 ( (Cat # AT3016a )
  • E - NEK2 Antibody (monoclonal) (M11) AT3016a
    Detection limit for recombinant GST tagged NEK2 is approximately 3ng/ml as a capture antibody.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P51955
Other Accession BC043502
Reactivity Human, Mouse, Rat
Host mouse
Clonality Monoclonal
Isotype IgG2a Kappa
Clone Names 3B7
Calculated MW 51763 Da
Additional Information
Gene ID 4751
Other Names Serine/threonine-protein kinase Nek2, HSPK 21, Never in mitosis A-related kinase 2, NimA-related protein kinase 2, NimA-like protein kinase 1, NEK2, NEK2A, NLK1
Target/Specificity NEK2 (AAH43502, 331 a.a. ~ 445 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsNEK2 Antibody (monoclonal) (M11) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)

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Involvement of a centrosomal protein kendrin in the maintenance of centrosome cohesion by modulating Nek2A kinase activity. Matsuo K, et al. Biochem Biophys Res Commun, 2010 Jul 23. PMID 20599736.The Ras oncogene signals centrosome amplification in mammary epithelial cells through cyclin D1/Cdk4 and Nek2. Zeng X, et al. Oncogene, 2010 Sep 9. PMID 20581865.Centrosome-related genes, genetic variation, and risk of breast cancer. Olson JE, et al. Breast Cancer Res Treat, 2010 May 28. PMID 20508983.Three genetic developmental stages of papillary renal cell tumors: duplication of chromosome 1q marks fatal progression. Szponar A, et al. Int J Cancer, 2009 May 1. PMID 19123481.Nek2 as a novel molecular target for the treatment of breast carcinoma. Tsunoda N, et al. Cancer Sci, 2009 Jan. PMID 19038001.

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$ 350.00
Cat# AT3016a
Availability: 7-10 days
Bulk Size

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