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NODAL Antibody (monoclonal) (M03)

Mouse monoclonal antibody raised against a partial recombinant NODAL.

     
  • IF - NODAL Antibody (monoclonal) (M03) AT3074a
    Immunofluorescence of monoclonal antibody to NODAL on HeLa cell. [antibody concentration 10 ug/ml]
    detail
  • WB - NODAL Antibody (monoclonal) (M03) AT3074a
    Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (33.66 KDa) .
    detail
  • WB - NODAL Antibody (monoclonal) (M03) AT3074a
    NODAL monoclonal antibody (M03), clone 5C3 Western Blot analysis of NODAL expression in HeLa ( (Cat # AT3074a )
    detail
  • WB - NODAL Antibody (monoclonal) (M03) AT3074a
    NODAL monoclonal antibody (M03), clone 5C3. Western Blot analysis of NODAL expression in PC-12 ( (Cat # AT3074a )
    detail
  • WB - NODAL Antibody (monoclonal) (M03) AT3074a
    NODAL monoclonal antibody (M03), clone 5C3. Western Blot analysis of NODAL expression in Raw 264.7 ( (Cat # AT3074a )
    detail
  • IHC - NODAL Antibody (monoclonal) (M03) AT3074a
    Immunoperoxidase of monoclonal antibody to NODAL on formalin-fixed paraffin-embedded human endometrium cancer. [antibody concentration 3 ug/ml]
    detail
  • E - NODAL Antibody (monoclonal) (M03) AT3074a
    Detection limit for recombinant GST tagged NODAL is approximately 0.3ng/ml as a capture antibody.
    detail
  • SPECIFICATION
  • CITATIONS
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, IF
Primary Accession Q96S42
Other Accession NM_018055
Reactivity Human, Mouse, Rat
Host mouse
Clonality Monoclonal
Isotype IgG1 Kappa
Clone Names 5C3
Calculated MW 39561 Da
Additional Information
Gene ID 4838
Other Names Nodal homolog, NODAL
Target/Specificity NODAL (NP_060525, 275 a.a. ~ 346 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Dilution WB~~1:500~1000
Format Clear, colorless solution in phosphate buffered saline, pH 7.2 .
StorageStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
PrecautionsNODAL Antibody (monoclonal) (M03) is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas
Citations (0)
citation

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Background

The protein encoded by this gene is a member of the TGF-beta superfamily. Studies of the mouse counterpart suggested that this gene may be essential for mesoderm formation and subsequent organization of axial structures in early embryonic development.

References

1.Reactivation of embryonic nodal signaling is associated with tumor progression and promotes the growth of prostate cancer cells.Lawrence MG, Margaryan NV, Loessner D, Collins A, Kerr KM, Turner M, Seftor EA, Stephens CR, Lai J, Postovit LM, Clements JA, Hendrix MJ; APC BioResource.Prostate. 2011 Jan 12. [Epub ahead of print]

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$ 350.00
Cat# AT3074a
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