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RAD18 Antibody (monoclonal) (M03)
Mouse monoclonal antibody raised against a partial recombinant RAD18.
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IF |
|---|---|
| Primary Accession | Q9NS91 |
| Other Accession | NM_020165 |
| Reactivity | Human, Rat |
| Host | mouse |
| Clonality | Monoclonal |
| Isotype | IgG2b Kappa |
| Clone Names | 2B9 |
| Calculated MW | 56223 Da |
| Gene ID | 56852 |
|---|---|
| Other Names | E3 ubiquitin-protein ligase RAD18, 632-, Postreplication repair protein RAD18, hHR18, hRAD18, RING finger protein 73, RAD18, RNF73 |
| Target/Specificity | RAD18 (NP_064550, 332 a.a. ~ 430 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
| Dilution | WB~~1:500~1000 |
| Format | Clear, colorless solution in phosphate buffered saline, pH 7.2 . |
| Storage | Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing. |
| Precautions | RAD18 Antibody (monoclonal) (M03) is for research use only and not for use in diagnostic or therapeutic procedures. |
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Provided below are standard protocols that you may find useful for product applications.
Background
The protein encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens.
References
A Large-scale genetic association study of esophageal adenocarcinoma risk. Liu CY, et al. Carcinogenesis, 2010 Jul. PMID 20453000.RAD18-dependent recruitment of SNM1A to DNA repair complexes by a ubiquitin-binding zinc finger. Yang K, et al. J Biol Chem, 2010 Jun 18. PMID 20385554.Multiple genetic variants in telomere pathway genes and breast cancer risk. Shen J, et al. Cancer Epidemiol Biomarkers Prev, 2010 Jan. PMID 20056641.Mutation analysis of Rad18 in human cancer cell lines and non small cell lung cancer tissues. Nakamura T, et al. J Exp Clin Cancer Res, 2009 Jul 25. PMID 19630985.Physical and functional interaction between WRNIP1 and RAD18. Yoshimura A, et al. Genes Genet Syst, 2009 Apr. PMID 19556710.
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