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APC Anti-Mouse CD80 (B7-1) (16-10A1) Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| FC |
|---|---|
| Isotype | Armenian Hamster IgG |
| Concentration | 0.2mg/ml |
| Reactivity | Mouse |
| Formulation | 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2 |
| Gene ID | 12519 |
|---|---|
| Gene Name | Cd80 |
| Alternative Name(s) | B7, Ly-53 |
| Format | APC |
| Storage Conditions | 2-8°C protected from light |
Provided below are standard protocols that you may find useful for product applications.
Background
The 16-10A1 antibody reacts with mouse CD80, also known as B7-1, a 55 kDa type I transmembrane protein ligand for CD152 (CTLA-4) and for CD28, a co-stimulatory receptor for the T cell receptor (TCR). CD28 also binds a second B7 ligand known as CD86 (B7-2). Both CD80 and CD86 are expressed on activated B cells and antigen-presenting cells. These ligands trigger CD28 signaling in concert with TCR activation to drive T cell proliferation, induce high-level expression of IL-2, impart resistance to apoptosis, and enhance T cell cytotoxicity. The interaction / co-stimulatory signaling between the B7 ligands and CD28 or CTLA-4 provides crucial communication between T cells and B cells or APCs to coordinate the adaptive immune response.
References
Thaventhiran JED, Hoffmann A, Magiera L, de la Roche M, Lingel H, Brunner-Weinzierl M, and Fearon DT. 2012. Proc. Natl. Acad. Sci. 10.1073. (in vitro blocking, Flow cytometry)
Liu Z, Geboes K, Hellings P, Maerten P, Heremans H, Vandenberghe P, Boon L, van Kooten P, Rutgeerts P, and Ceuppens JL. 2011. J. Immunol. 167: 1830-1838. (in vivo blocking, Immunohistochemistry – OCT embedded frozen tissue)
Anraku M, Tagawa T, Wu Licun, Yun Z, Keshavjee S, Zhang L, Johnston MR, and de Perrot M. 2010. J. Immunol. 185:956-966. (Flow cytometry)
Odobasic D, Kitching AR, Semple TJ, Timoshanko JR, Tipping PG, and Holdsworth SR. 2005. J. Am. Soc. Nephrol. 16: 2012-2022. (in vivo activation, Immunofluorescence microscopy and Immunohistochemistry – frozen tissue)
Lenschow DJ, Ho SC, Sattar H, Rhee L, Gray G, Nabavi N, Herold KC, and Bluestone JA. 1995. J. Exp. Med. 181:1145-155. (in vitro blocking)
Razi-Wold Z, Freeman GJ, Galvin F, Benacerraf B, Nadler L, and Reiser H. 1992. Proc. Natl. Acad. Sci. 89:4210-4214. (Origination of clone, Immunoprecipitation, in vitro blocking)
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