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FAM50A Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • IF - FAM50A Antibody (C-term) AW5115-U100
    Fluorescent image of Hela cells stained with FAM50A Antibody (C-term)(Cat#AW5115). AW5115 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
    detail
  • WB - FAM50A Antibody (C-term) AW5115-U100
    Western blot analysis of lysates from A549,Hela,MDA-MB-453 cell line (from left to right), using FAM50AAntibody(C-term)(Cat. #AW5115). AW5115 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.Lysates at 20ug per lane.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IF, WB
Primary Accession Q14320
Other Accession Q9WV03, Q568K9, Q4KLV7, Q2VPH1
Reactivity Human
Predicted Xenopus, Zebrafish, Mouse
Host Rabbit
Clonality Polyclonal
Calculated MW H=40;M=40 KDa
Isotype Rabbit IgG
Antigen Source Human
Additional Information
Gene ID 9130
Antigen Region 311-339 aa
Other Names FAM50A;DXS9928E; HXC26; XAP5; Protein FAM50A; Protein FAM50A; Protein HXC-26; Protein FAM50A; Protein XAP-5
Dilution IF~~1:25
WB~~1:1000
Target/Specificity This FAM50A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 311-339 amino acids from the C-terminal region of human FAM50A.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsFAM50A Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name FAM50A
Synonyms DXS9928E, HXC26, XAP5
Function Probably involved in the regulation of pre-mRNA splicing.
Cellular Location Nucleus.
Tissue Location Widely expressed in fetal and adult tissues. Mostly abundant in fetal brain, liver and kidney; in the adult, high levels were also observed in heart, skeletal muscle, spleen, thymus, prostate and small intestine. Expressed in fetal cerebellum and hypothalamus Low expression is observed in fetal temporal lobe (PubMed:32703943)
Citations (0)
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Background

May be a DNA-binding protein or transcriptional factor.

References

Mazzarella R., et al. Genomics 45:216-219(1997).
Ota T., et al. Nat. Genet. 36:40-45(2004).
Ross M.T., et al. Nature 434:325-337(2005).
Mural R.J., et al. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
Toyoda A., et al. DNA Res. 3:337-340(1996).

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$ 370.00
Cat# AW5115-U100
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