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(Mouse) Scx Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - (Mouse) Scx Antibody (N-term) AW5469-U100
    All lanes : Anti-Scx Antibody (N-term) at 1:1000 dilution Lane 1: mouse lung lysates Lane 2: mouse liver lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 22 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
    detail
  • IHC-P - (Mouse) Scx Antibody (N-term) AW5469-U100
    AW5469 staining Scx in Mouse lung tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
    detail
  • SPECIFICATION
  • CITATIONS: 1
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P
Primary Accession Q64124
Other Accession Q7RTU7
Reactivity Mouse
Predicted Human
Host Rabbit
Clonality Polyclonal
Calculated MW M=22;H=22 KDa
Isotype Rabbit IgG
Antigen Source HUMAN
Additional Information
Gene ID 20289
Antigen Region 69-102 aa
Other Names Basic helix-loop-helix transcription factor scleraxis, Scx
Dilution WB~~1:1000
IHC-P~~1:25
Target/Specificity This mouse Scx antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 69-102 amino acids from the N-terminal region of mouse Scx.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions(Mouse) Scx Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Scx
Function Plays an early essential role in mesoderm formation, as well as a later role in formation of somite-derived chondrogenic lineages.
Cellular Location Nucleus {ECO:0000255|PROSITE-ProRule:PRU00981}.
Tissue Location Expressed in mesenchymal precursors of cartilage and in connective tissue. Highly expressed in tendons in the limb, tongue and diaphragm and in cartilage of the bronchi
Research Areas
Citations ( 0 )

Background

Plays an early essential role in mesoderm formation, as well as a later role in formation of somite-derived chondrogenic lineages.

References

Cserjesi P.,et al.Development 121:1099-1110(1995).
Brown D.,et al.Development 126:4317-4329(1999).

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$ 370.00
Cat# AW5469-U100
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