PRKAG3 Antibody (C-term) Blocking Peptide
Synthetic peptide
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | Q9UGI9 |
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Clone Names | 100617193 |
Gene ID | 53632 |
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Other Names | 5'-AMP-activated protein kinase subunit gamma-3, AMPK gamma3, AMPK subunit gamma-3, PRKAG3, AMPKG3 |
Format | Peptides are lyophilized in a solid powder format. Peptides can be reconstituted in solution using the appropriate buffer as needed. |
Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. |
Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
Name | PRKAG3 |
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Synonyms | AMPKG3 |
Function | AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism (PubMed:14722619, PubMed:24563466, PubMed:17878938). In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. AMPK also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. The AMPK gamma3 subunit is a non-catalytic subunit with a regulatory role in muscle energy metabolism (PubMed:17878938). It mediates binding to AMP, ADP and ATP, leading to AMPK activation or inhibition: AMP-binding results in allosteric activation of alpha catalytic subunit (PRKAA1 or PRKAA2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits. ADP also stimulates phosphorylation, without stimulating already phosphorylated catalytic subunit. ATP promotes dephosphorylation of catalytic subunit, rendering the AMPK enzyme inactive. |
Tissue Location | Skeletal muscle, with weak expression in heart and pancreas |
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Provided below are standard protocols that you may find useful for product applications.
Background
The protein encoded by this gene is a regulatory subunitof the AMP-activated protein kinase (AMPK). AMPK is a heterotrimerconsisting of an alpha catalytic subunit, and non-catalytic betaand gamma subunits. AMPK is an important energy-sensing enzyme thatmonitors cellular energy status. In response to cellular metabolicstresses, AMPK is activated, and thus phosphorylates andinactivates acetyl-CoA carboxylase (ACC) and beta-hydroxybeta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved inregulating de novo biosynthesis of fatty acid and cholesterol. Thissubunit is one of the gamma regulatory subunits of AMPK. It isdominantly expressed in skeletal muscle. Studies of the pigcounterpart suggest that this subunit may play a key role in theregulation of energy metabolism in skeletal muscle. [provided byRefSeq].
References
Jablonski, K.A., et al. Diabetes 59(10):2672-2681(2010)Jassim, G., et al. Pharmacopsychiatry (2010) In press :Crawford, S.A., et al. Diabetologia 53(9):1986-1997(2010)Ramanathan, L., et al. Protein Expr. Purif. 70(1):13-22(2010)McGeachie, M., et al. Circulation 120(24):2448-2454(2009)
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