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SMURF1 Antibody (C-term) Blocking Peptide
Synthetic peptide
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Primary Accession | Q9HCE7 |
|---|---|
| Clone Names | 3060612 |
| Gene ID | 57154 |
|---|---|
| Other Names | E3 ubiquitin-protein ligase SMURF1, hSMURF1, 632-, SMAD ubiquitination regulatory factor 1, SMAD-specific E3 ubiquitin-protein ligase 1, SMURF1, KIAA1625 |
| Target/Specificity | The synthetic peptide sequence used to generate the antibody AP2104b was selected from the C-term region of human SMURF1 . A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay. |
| Format | Peptides are lyophilized in a solid powder format. Peptides can be reconstituted in solution using the appropriate buffer as needed. |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Name | SMURF1 |
|---|---|
| Synonyms | KIAA1625 |
| Function | E3 ubiquitin-protein ligase that acts as a negative regulator of BMP signaling pathway. Mediates ubiquitination and degradation of SMAD1 and SMAD5, 2 receptor-regulated SMADs specific for the BMP pathway. Promotes ubiquitination and subsequent proteasomal degradation of TRAF family members and RHOA. Promotes ubiquitination and subsequent proteasomal degradation of MAVS (PubMed:23087404). Acts as an antagonist of TGF-beta signaling by ubiquitinating TGFBR1 and targeting it for degradation (PubMed:21791611). Plays a role in dendrite formation by melanocytes (PubMed:23999003). |
| Cellular Location | Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side |
| Tissue Location | Expressed in melanocytes (PubMed:23999003). |
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Provided below are standard protocols that you may find useful for product applications.
Background
SMURF1 is an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. This protein interacts with receptor-regulated SMADs specific for the BMP pathway, SMAD1 and SMAD5, in order to trigger their ubiquitination and degradation and thereby their inactivation.
References
Tajima, Y., et al., J. Biol. Chem. 278(12):10716-10721 (2003).Suzuki, C., et al., J. Biol. Chem. 277(42):39919-39925 (2002).Ebisawa, T., et al., J. Biol. Chem. 276(16):12477-12480 (2001).Zhu, H., et al., Nature 400(6745):687-693 (1999).Lambris, J., et al., J. Immunol. Methods 27(1):55-59 (1979).
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