MGRN1 Antibody (N-term) Blocking Peptide
Synthetic peptide
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | O60291 |
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Clone Names | 80917049 |
Gene ID | 23295 |
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Other Names | E3 ubiquitin-protein ligase MGRN1, 632-, Mahogunin RING finger protein 1, RING finger protein 156, MGRN1, KIAA0544, RNF156 |
Target/Specificity | The synthetic peptide sequence used to generate the antibody AP7634a was selected from the N-term region of human MGRN1. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay. |
Format | Peptides are lyophilized in a solid powder format. Peptides can be reconstituted in solution using the appropriate buffer as needed. |
Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. |
Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
Name | MGRN1 |
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Synonyms | KIAA0544, RNF156 |
Function | E3 ubiquitin-protein ligase. Mediates monoubiquitination at multiple sites of TSG101 in the presence of UBE2D1, but not of UBE2G1, nor UBE2H. Plays a role in the regulation of endosome-to-lysosome trafficking. Impairs MC1R- and MC4R-signaling by competing with GNAS- binding to MCRs and inhibiting agonist-induced cAMP production. Does not inhibit ADRB2-signaling. Does not promote MC1R ubiquitination. Acts also as a negative regulator of hedgehog signaling (By similarity). |
Cellular Location | Early endosome. Note=The endosomal localization is dependent on the interaction with TSG101 [Isoform 2]: Cytoplasm, cytosol. Nucleus. Note=Translocation from the cytosol to the nucleus is seen only in the presence of MC1R and MC4R, but not TBXA2R. Excluded from nucleoli [Isoform 4]: Cytoplasm, cytosol. Cell membrane. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Mahogunin (MGRN1) is a C3HC4 RING-containing protein with E3 ubiquitin ligase activity in vitro.
References
Chakrabarti,O., Cell 137 (6), 1136-1147 (2009)Kim,B.Y., Mol. Biol. Cell 18 (4), 1129-1142 (2007)
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