CYP4X1 Antibody (N-term) Blocking Peptide
Synthetic peptide
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | Q8N118 |
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Clone Names | 80311143 |
Gene ID | 260293 |
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Other Names | Cytochrome P450 4X1, CYPIVX1, CYP4X1 |
Target/Specificity | The synthetic peptide sequence used to generate the antibody AP7885a was selected from the N-term region of human CYP4X1. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay. |
Format | Peptides are lyophilized in a solid powder format. Peptides can be reconstituted in solution using the appropriate buffer as needed. |
Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. |
Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
Name | CYP4X1 {ECO:0000303|PubMed:18549450, ECO:0000312|HGNC:HGNC:20244} |
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Function | A cytochrome P450 monooxygenase that selectively catalyzes the epoxidation of the last double bond of the arachidonoyl moiety of anandamide, potentially modulating endocannabinoid signaling. Has no hydroxylase activity toward various fatty acids, steroids and prostaglandins. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase). |
Cellular Location | Endoplasmic reticulum membrane {ECO:0000250|UniProtKB:Q6A152}; Single-pass membrane protein. Microsome membrane {ECO:0000250|UniProtKB:Q6A152}; Single-pass membrane protein |
Tissue Location | Expressed in brain, heart, kidney and skin and, at lower levels, in skeletal muscle and liver (PubMed:16478468, PubMed:18549450). In the brain, high levels are detected in amygdala and lower levels in globus pallidus and cerebellum (PubMed:18549450) In the heart, very high levels in aorta, but very low levels in other heart regions (PubMed:16478468, PubMed:18549450). Also expressed in breast, prostate and colon (PubMed:18549450) |
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Provided below are standard protocols that you may find useful for product applications.
Background
CYP4X1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The expression pattern of a similar rat protein suggests that this protein may be involved in neurovascular function in the brain.
References
Savas,U., Arch. Biochem. Biophys. 436 (2), 377-385 (2005)Nelson,D.R., Pharmacogenetics 14 (1), 1-18 (2004)Bylund,J., Biochem. Biophys. Res. Commun. 296 (3), 677-684 (2002)
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