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BEST2 Antibody (C-term) Blocking Peptide
Synthetic peptide
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
| Primary Accession | Q8NFU1 |
|---|---|
| Clone Names | 91204007 |
| Gene ID | 54831 |
|---|---|
| Other Names | Bestrophin-2, Vitelliform macular dystrophy 2-like protein 1, BEST2, VMD2L1 |
| Target/Specificity | The synthetic peptide sequence used to generate the antibody AP9246b was selected from the C-term region of human BEST2. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay. |
| Format | Peptides are lyophilized in a solid powder format. Peptides can be reconstituted in solution using the appropriate buffer as needed. |
| Storage | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C. |
| Precautions | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Name | BEST2 (HGNC:17107) |
|---|---|
| Synonyms | VMD2L1 |
| Function | Ligand-gated anion channel that allows the movement of anions across cell membranes when activated by calcium (Ca2+) (PubMed:11904445, PubMed:18400985, PubMed:32251414, PubMed:35789156, PubMed:36289327). Transports a large specter of anions, namely mediates the movement of chloride, L-glutamate and iodide (PubMed:11904445, PubMed:18400985, PubMed:32251414, PubMed:35789156, PubMed:36289327). Calcium-binding triggers the dilation of the aperture, but calcium- dependent gating is only effective when the size of the passing anion is bigger than the closed aperture (By similarity). Mediates the calcium-activated hydrogencarbonate movement and participates in colonic hydrogencarbonate secretion concomitant with mucin secretion (By similarity). In non-pigmented epithelium (NPE), mediates the efflux of intracellular L-glutamate; binding of intracellular L-glutamate activates and open both the neck and the aperture of the channel, leading to L-glutamate exit promoting chloride influx movement from the extracellular side in trans (PubMed:36289327). Also exhibits a directional permeability for intracellular glutamine, in a similar manner as for L-glutamate (PubMed:36289327). |
| Cellular Location | Cell membrane {ECO:0000250|UniProtKB:E1BF86}; Multi-pass membrane protein. Basolateral cell membrane; Multi-pass membrane protein |
| Tissue Location | Mainly confined to the retinal pigment epithelium (PubMed:12032738). Expressed in colon (PubMed:12032738, PubMed:20407206). |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
info@abcepta.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Background
BEST2 is a member of the bestrophin gene family of anion channels. Bestrophin genes share a similar gene structure with highly conserved exon-intron boundaries, but with distinct 3' ends. Bestrophins are transmembrane proteins that contain a homologous region rich in aromatic residues, including an invariant arg-phe-pro motif.
References
Zhang,Y., et.al, Mol. Vis. 16, 200-206 (2010)Marsey,L.L. et.al, J. Physiol. (Lond.) 587 (PT 10), 2211-2224 (2009)
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