Human recombinant protein UBE2E2 (UbcH8)
Human recombinant UBE2E2 (UbcH8)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Primary Accession | Q96LR5 |
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Calculated MW | 22.255 kDa |
Gene ID | 7325 |
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Gene Symbol | UBE2E2 |
Other Names | RIG-B, UBCH8, MGC40331, UBE2L6, Ubiquitin/ISG15-conjugating enzyme E2 L6, Ubiquitin-protein ligase L6, Ubiquitin carrier protein L6, and Retinoic acid-induced gene B protein. |
Gene Source | Human |
Assay&Purity | RP-HPLC; ≥95% |
Assay2&Purity2 | N/A; |
Recombinant | Yes |
Format | Liquid |
Storage | -80°C; 75 µL of a 40 µM solution (3 nmoles) in 20 mM Tris, pH 7.4, 150 mM NaCl, 10 mM DTT and 10% glycerol. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Ubiquitin-conjugating enzyme E2 (UBE2E2) is a protein that in humans is encoded by the UBE2E2 gene. Amino acid sequence within the UBC domain of UBE2E2 shares over 90% identity with human UbcH6, mouse UbcM2, and Drosophila UbcD2, whereas the N-terminal region shows little amino acid sequence similarity with known proteins. The UBE2E2 protein forms a thioester bond with ubiquitin in an E1-dependent manner and mediates the transfer of ubiquitin from a ubiquitin-activating enzyme (E1) to a substrate protein or E3 ligase.
References
Kimura M.,et al.Cytogenet. Cell Genet. 78:107-111(1997).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Gauci S.,et al.Anal. Chem. 81:4493-4501(2009).
Mayya V.,et al.Sci. Signal. 2:RA46-RA46(2009).
David Y.,et al.J. Biol. Chem. 285:8595-8604(2010).
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