CTACK, Murine recombinant protein
Cutaneous T-cell Attracting Chemokine, CCL27, ALP, Skinkine, Eskine
|Calculated MW||10.9 kDa|
|Other Names||Cutaneous T-cell Attracting Chemokine, CCL27, ALP, Skinkine, Eskine|
|Sequence||LPLPSSTSCC TQLYRQPLPS RLLRRIVHME LQEADGDCHL QAVVLHLARR SVCVHPQNRS LARWLERQGK RLQGTVPSLN LVLQKKMYSN PQQQN|
|Application Notes||Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.|
|Storage||-20°C; Sterile filtered through a 0.2 micron filter. Lyophilized with no additives.|
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Provided below are standard protocols that you may find useful for product applications.
CTACK is a keratinocyte-derived CC chemokine which signals through the CCR10 receptor. Both CTACK and CCR10 are expressed in normal and irritated epithelial cells. CTACK selectively attracts CLA+ T-cells and directs them into the skin. CTACK contains the four highly conserved cysteine residues present in most CC chemokines. The mature protein contains 88 amino acid residues. Recombinant murine CTACK is a 10.9 kDa Protein, containing 95 amino acid residues.
Hromas R.,et al.Biochem. Biophys. Res. Commun. 258:737-740(1999).
Ishikawa-Mochizuki I.,et al.FEBS Lett. 460:544-548(1999).
Morales J.,et al.Proc. Natl. Acad. Sci. U.S.A. 96:14470-14475(1999).
Carninci P.,et al.Science 309:1559-1563(2005).
Baird J.W.,et al.J. Biol. Chem. 274:33496-33503(1999).
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