Anti-MPI Picoband Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC, IHC-P, IHC-F, ICC, FC |
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Primary Accession | P34949 |
Host | Rabbit |
Reactivity | Human, Mouse, Rat |
Clonality | Polyclonal |
Format | Lyophilized |
Description | Rabbit IgG polyclonal antibody for Mannose-6-phosphate isomerase(MPI) detection. Tested with WB in Human;Rat. |
Reconstitution | Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Gene ID | 4351 |
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Other Names | Mannose-6-phosphate isomerase, 5.3.1.8, Phosphohexomutase, Phosphomannose isomerase, PMI, MPI, PMI1 |
Calculated MW | 46656 MW KDa |
Application Details | Western blot, 0.1-0.5 µg/ml, Human, Rat |
Subcellular Localization | Cytoplasm . |
Tissue Specificity | Expressed in all tissues, but more abundant in heart, brain and skeletal muscle. |
Protein Name | Mannose-6-phosphate isomerase |
Contents | Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. |
Immunogen | E. coli-derived human MPI recombinant protein (Position: A2-K99). Human MPI shares 88.8% and 86.7% amino acid (aa) sequence identity with mouse and rat MPI, respectively. |
Purification | Immunogen affinity purified. |
Cross Reactivity | No cross reactivity with other proteins. |
Storage | At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing. |
Name | MPI (HGNC:7216) |
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Synonyms | PMI1 |
Function | Isomerase that catalyzes the interconversion of fructose-6-P and mannose-6-P and has a critical role in the supply of D-mannose derivatives required for many eukaryotic glycosylation reactions. |
Cellular Location | Cytoplasm {ECO:0000250|UniProtKB:Q924M7}. |
Tissue Location | Expressed in all tissues, but more abundant in heart, brain and skeletal muscle. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Mannose-6 phosphate isomerase (MPI), alternately phosphomannose isomerase (PMI), is an enzyme which facilitates the interconversion of fructose 6-phosphate(F6P) and mannose-6-phosphate(M6P). It also plays a critical role in maintaining the supply of D-mannose derivatives, which are required for most glycosylation reactions. Mutations in the MPI gene were found in patients with carbohydrate-deficient glycoprotein syndrome, type Ib. Alternative splicing results in multiple transcript variants. This MPI gene is mapped to 15q24.1.
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