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Goat Anti-IRF4 (aa393-407) Antibody

Peptide-affinity purified goat antibody

     
  • WB - Goat Anti-IRF4 (aa393-407) Antibody AF4335a
    EB11331 (0.3µg/ml) staining of Human (A) and (0.1ug/ml) Rat (B) Spleen lysate (35µg protein in RIPA buffer).
    detail
  • IHC - Goat Anti-IRF4 (aa393-407) Antibody AF4335a
    EB11331 (5µg/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
    detail
  • FC - Goat Anti-IRF4 (aa393-407) Antibody AF4335a
    EB11331 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) fo
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC, FC, Pep-ELISA
Primary Accession Q15306
Other Accession NP_002451.2, NP_001182215.1
Reactivity Human, Rat
Host Goat
Clonality Polyclonal
Clone Names IRF4
Calculated MW 51772 Da
Additional Information
Gene ID 3662
Other Names interferon regulatory factor 4, LSIRF, multiple myeloma oncogene 1, MUM1, IRF4
Format Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Immunogen Peptide with sequence C-DPQRQRKLITAHVEP, from the internal region (near C Terminus) of the protein sequence according to NP_002451.2; NP_001182215.1.
StorageMaintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGoat Anti-IRF4 (aa393-407) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name IRF4
Synonyms MUM1
Function Transcriptional activator. Binds to the interferon-stimulated response element (ISRE) of the MHC class I promoter. Binds the immunoglobulin lambda light chain enhancer, together with PU.1. Probably plays a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells. Involved in CD8(+) dendritic cell differentiation by forming a complex with the BATF-JUNB heterodimer in immune cells, leading to recognition of AICE sequence (5'-TGAnTCA/GAAA- 3'), an immune-specific regulatory element, followed by cooperative binding of BATF and IRF4 and activation of genes (By similarity).
Cellular Location Nucleus.
Tissue Location Lymphoid cells.
Research Areas
Citations (0)
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$ 341.00
Cat# AF4335a
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Availability: 7-10 days
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