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KD-Validated Anti-CDKN2C Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-CDKN2C Rabbit Monoclonal Antibody AGI1022
    Western blotting analysis using anti-CDKN2C antibody (Cat#AGI1022). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CDKN2C antibody (Cat#AGI1022, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-CDKN2C Rabbit Monoclonal Antibody AGI1022
    Western blotting analysis using anti-CDKN2C antibody (Cat#AGI1022). CDKN2C expression in wild type (WT) and CDKN2C shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-CDKN2C antibody (Cat#AGI1022, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • FC - KD-Validated Anti-CDKN2C Rabbit Monoclonal Antibody AGI1022
    Flow cytometric analysis of CDKN2C expression in HeLa cells using CDKN2C antibody(Cat#AGI1022, 1:2,000). Green, isotype control; red, CDKN2C.
    detail
  • ICC - KD-Validated Anti-CDKN2C Rabbit Monoclonal Antibody AGI1022
    Immunocytochemical staining of Hela cells with anti-CDKN2C antibody (Cat#AGI1022, 1:1,000). Nuclei were stained blue with DAPI; CDKN2C was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession P42773
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 24GB5945
Calculated MW Predicted, 18 kDa, observed, 15 kDa
Gene Name CDKN2C
Aliases CDKN2C; Cyclin Dependent Kinase Inhibitor 2C; INK4C; P18; Cyclin-Dependent Kinase Inhibitor 2C (P18, Inhibits CDK4); Cyclin-Dependent Kinase 4 Inhibitor C; P18-INK6; Cyclin-Dependent Kinase 6 Inhibitor P18; Cyclin-Dependent Kinase 6 Inhibitor; Cyclin-Dependent Inhibitor; CDK6 Inhibitor P18; P18-INK4C; P18-INK4c; CDKN6
Immunogen A synthesized peptide derived from human CDKN2C
Additional Information
Gene ID 1031
Other Names Cyclin-dependent kinase 4 inhibitor C, Cyclin-dependent kinase 6 inhibitor, p18-INK4c, p18-INK6, CDKN2C, CDKN6
Protein Information
Name CDKN2C
Synonyms CDKN6
Function Interacts strongly with CDK6, weakly with CDK4. Inhibits cell growth and proliferation with a correlated dependence on endogenous retinoblastoma protein RB.
Tissue Location Highest levels found in skeletal muscle. Also found in pancreas and heart
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI1022
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