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KD-Validated Anti-Kinesin light chain 1 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-Kinesin light chain 1 Rabbit Monoclonal Antibody AGI1044
    Western blotting analysis using anti-kinesin light chain 1 antibody (Cat#AGI1044). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-kinesin light chain 1 antibody (Cat#AGI1044, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-Kinesin light chain 1 Rabbit Monoclonal Antibody AGI1044
    Western blotting analysis using anti-kinesin light chain 1 antibody (Cat#AGI1044). Kinesin light chain 1 expression in wild type (WT) and kinesin light chain 1 (KLC1) shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-kinesin light chain 1 antibody (Cat#AGI1044, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • FC - KD-Validated Anti-Kinesin light chain 1 Rabbit Monoclonal Antibody AGI1044
    Flow cytometric analysis of kinesin light chain 1 expression in HepG2 cells using kinesin light chain 1 antibody (Cat#AGI1044, 1:2,000). Green, isotype control; red, kinesin light chain 1.
    detail
  • ICC - KD-Validated Anti-Kinesin light chain 1 Rabbit Monoclonal Antibody AGI1044
    Immunocytochemical staining of HepG2 cells with anti-kinesin light chain 1 antibody (Cat#AGI1044, 1:1,000). Nuclei were stained blue with DAPI; Kinesin light chain 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession Q07866
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 24GB6030
Calculated MW Predicted, 65 kDa, observed, 65 kDa
Gene Name KLC1
Aliases KLC1; Kinesin Light Chain 1; KLC; KNS2A; KNS2; Kinesin 2 60/70kDa; HKLC1S; HKLC1N; HKLC1P; HKLC1G; HKLC1R; HKLC1J; HKLC1B; Medulloblastoma Antigen MU-MB-2.50; Kinesin 2; KLC
Immunogen A synthesized peptide derived from human KLC1
Additional Information
Gene ID 3831
Other Names Kinesin light chain 1, KLC 1, KLC1, KLC, KNS2
Protein Information
Name KLC1
Synonyms KLC, KNS2
Function Kinesin is a microtubule-associated force-producing protein that may play a role in organelle transport (PubMed:21385839). The light chain may function in coupling of cargo to the heavy chain or in the modulation of its ATPase activity (By similarity).
Cellular Location Cell projection, growth cone {ECO:0000250|UniProtKB:P37285}. Cytoplasmic vesicle. Cytoplasm, cytoskeleton
Tissue Location Found in a variety of tissues. Mostly abundant in brain and spine.
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI1044
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