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KD-Validated Anti-Catalase Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-Catalase Rabbit Monoclonal Antibody AGI1143
    Western blotting analysis using anti-Catalase antibody (Cat#AGI1143). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Catalase antibody (Cat#AGI1143, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-Catalase Rabbit Monoclonal Antibody AGI1143
    Western blotting analysis using anti-Catalase antibody (Cat#AGI1143). Catalase expression in wild type (WT) and Catalase shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Catalase antibody (Cat#AGI1143, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • FC - KD-Validated Anti-Catalase Rabbit Monoclonal Antibody AGI1143
    Flow cytometric analysis of Catalase expression in HepG2 cells using anti-Catalase antibody (Cat #AGI1143, 1:2,000). Green, isotype control; red, Catalase.
    detail
  • ICC - KD-Validated Anti-Catalase Rabbit Monoclonal Antibody AGI1143
    Immunocytochemical staining of HepG2 cells with anti-Catalase antibody (Cat#AGI1143, 1:1,000). Nuclei were stained blue with DAPI; Catalase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession P04040
Reactivity Rat, Human
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 23GB 2625
Calculated MW Predicted, 60 kDa , observed, 60 kDa
Gene Name CAT
Aliases CAT; Catalase; EC 1.11.1.6; Epididymis Secretory Sperm Binding Protein
Immunogen A synthesized peptide derived from human Catalase
Additional Information
Gene ID 847
Other Names Catalase, 1.11.1.6, CAT
Protein Information
Name CAT
Function Catalyzes the degradation of hydrogen peroxide (H(2)O(2)) generated by peroxisomal oxidases to water and oxygen, thereby protecting cells from the toxic effects of hydrogen peroxide (PubMed:7882369). Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells (PubMed:7882369).
Cellular Location Peroxisome matrix
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI1143
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