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KD-Validated Anti-CDC25C Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-CDC25C Rabbit Monoclonal Antibody AGI1184
    Western blotting analysis using anti-CDC25C antibody (Cat#61384). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CDC25C antibody (Cat#61384, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • WB - KD-Validated Anti-CDC25C Rabbit Monoclonal Antibody AGI1184
    Western blotting analysis using anti-CDC25C antibody (Cat#61384). CDC25C expression in wild type (WT) and CDC25C shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-CDC25C antibody (Cat#61384, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • FC - KD-Validated Anti-CDC25C Rabbit Monoclonal Antibody AGI1184
    Flow cytometric analysis of CDC25C expression in HeLa cells using CDC25C antibody (Cat#61384, 1:2,000). Green, isotype control; red, CDC25C.
    detail
  • ICC - KD-Validated Anti-CDC25C Rabbit Monoclonal Antibody AGI1184
    Immunocytochemical staining of Hela cells with CDC25C antibody (Cat#61384, 1:1,000). Nuclei were stained blue with DAPI; CDC25C was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession P30307
Reactivity Human
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 23GB3430
Calculated MW Predicted, 53 kDa , observed, 60 kDa
Gene Name CDC25C
Aliases CDC25C; Cell Division Cycle 25C; M-Phase Inducer Phosphatase 3; PPP1R60; Protein Phosphatase 1, Regulatory Subunit 60; EC 3.1.3.48; CDC25; Dual Specificity Phosphatase CDC25C; Dual Specificity Phosphatase Cdc25C; Phosphotyrosine Phosphatase; CDC25 Homolog C (S. Pombe); Mitosis Inducer CDC25; CDC25 Homolog C
Immunogen A synthesized peptide derived from human Cdc25C
Additional Information
Gene ID 995
Other Names M-phase inducer phosphatase 3, 3.1.3.48, Dual specificity phosphatase Cdc25C, CDC25C
Protein Information
Name CDC25C
Function Functions as a dosage-dependent inducer in mitotic control. Tyrosine protein phosphatase required for progression of the cell cycle (PubMed:8119945). When phosphorylated, highly effective in activating G2 cells into prophase (PubMed:8119945). Directly dephosphorylates CDK1 and activates its kinase activity (PubMed:8119945).
Cellular Location Nucleus
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI1184
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