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KD-Validated Anti-Catechol-O-methyltransferase Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-Catechol-O-methyltransferase Rabbit Monoclonal Antibody AGI1224
    Western blotting analysis using anti-Catechol-O-methyltransferase antibody (Cat#AGI1224). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Catechol-O-methyltransferase antibody (Cat#AGI1224, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-Catechol-O-methyltransferase Rabbit Monoclonal Antibody AGI1224
    Western blotting analysis using anti-Catechol-O-methyltransferase antibody (Cat#AGI1224). Catechol-O-methyltransferase expression in wild type (WT) and Catechol-O-methyltransferase shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Catechol-O-methyltransferase antibody (Cat#AGI1224, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • FC - KD-Validated Anti-Catechol-O-methyltransferase Rabbit Monoclonal Antibody AGI1224
    Flow cytometric analysis of Catechol-O-methyltransferase expression in HT-1080 cells using Catechol-O-methyltransferase antibody (Cat#AGI1224, 1:2,000). Green, isotype control; red, Catechol-O-methyltransferase.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC
Primary Accession P21964
Reactivity Rat, Human
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 23GB3735
Calculated MW Predicted, 30 kDa , observed, 24,28 kDa
Gene Name COMT
Aliases COMT; Catechol-O-Methyltransferase; Catechol O-Methyltransferase; EC 2.1.1.6; Epididymis Secretory Sperm Binding Protein Li 98n; Testicular Tissue Protein Li 42; HEL-S-98n
Immunogen A synthesized peptide derived from human COMT
Additional Information
Gene ID 1312
Other Names Catechol O-methyltransferase, 2.1.1.6, COMT (HGNC:2228)
Protein Information
Name COMT (HGNC:2228)
Function Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol.
Cellular Location [Isoform Soluble]: Cytoplasm
Tissue Location Brain, liver, placenta, lymphocytes and erythrocytes
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI1224
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