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KD-Validated Anti-DDAH1 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-DDAH1 Rabbit Monoclonal Antibody AGI1337
    Western blotting analysis using anti-dimethylarginine dimethylaminohydrolase 1 antibody (Cat#AGI1337). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-dimethylarginine dimethylaminohydrolase 1 antibody (Cat#AGI1337, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-DDAH1 Rabbit Monoclonal Antibody AGI1337
    Western blotting analysis using anti-dimethylarginine dimethylaminohydrolase 1 antibody (Cat#AGI1337). Dimethylarginine dimethylaminohydrolase 1 expression in wild type (WT) and dimethylarginine dimethylaminohydrolase 1 (DDAH1) knockdown (KD) HSHC cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-dimethylarginine dimethylaminohydrolase 1 antibody (Cat#AGI1337, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • FC - KD-Validated Anti-DDAH1 Rabbit Monoclonal Antibody AGI1337
    Flow cytometric analysis of Dimethylarginine dimethylaminohydrolase 1 expression in HT-1080 cells using anti-Dimethylarginine dimethylaminohydrolase 1 antibody (Cat#AGI1337, 1:2,000). Green, isotype control; red, Dimethylarginine dimethylaminohydrolase 1.
    detail
  • ICC - KD-Validated Anti-DDAH1 Rabbit Monoclonal Antibody AGI1337
    Immunocytochemical staining of HT-1080 cells with anti-Dimethylarginine dimethylaminohydrolase 1 antibody (Cat#AGI1337, 1:1,000). Nuclei were stained blue with DAPI; Dimethylarginine dimethylaminohydrolase 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 µm.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession O94760
Reactivity Human
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 25GB2995
Calculated MW Predicted, 31 kDa; observed, 36 kDa
Gene Name DDAH1
Aliases DDAH1; Dimethylarginine Dimethylaminohydrolase 1; DDAH; N(G),N(G)-Dimethylarginine Dimethylaminohydrolase 1; Dimethylargininase-1; EC 3.5.3.18; DDAH-1; DDAHI; NG, NG-Dimethylarginine Dimethylaminohydrolase; Epididymis Secretory Protein Li 16; HEL-S-16
Immunogen A synthesized peptide derived from human DDAH1
Additional Information
Gene ID 23576
Other Names N(G), N(G)-dimethylarginine dimethylaminohydrolase 1, DDAH-1, Dimethylarginine dimethylaminohydrolase 1, 3.5.3.18, DDAHI, Dimethylargininase-1, DDAH1 (HGNC:2715), DDAH
Protein Information
Name DDAH1 (HGNC:2715)
Synonyms DDAH
Function Hydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)- monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation.
Tissue Location Detected in brain, liver, kidney and pancreas, and at low levels in skeletal muscle.
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI1337
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