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KD-Validated Anti-Rho GTPase activating protein 5 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-Rho GTPase activating protein 5 Rabbit Monoclonal Antibody AGI1361
    Western blotting analysis using anti-Rho GTPase activating protein 5 antibody (Cat#AGI1361). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Rho GTPase activating protein 5 antibody (Cat#AGI1361, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-Rho GTPase activating protein 5 Rabbit Monoclonal Antibody AGI1361
    Western blotting analysis using anti-Rho GTPase activating protein 5 antibody (Cat#AGI1361). Rho GTPase activating protein 5 expression in wild type (WT) and Rho GTPase activating protein 5 shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Rho GTPase activating protein 5 antibody (Cat#AGI1361, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • FC - KD-Validated Anti-Rho GTPase activating protein 5 Rabbit Monoclonal Antibody AGI1361
    Flow cytometric analysis of Rho GTPase activating protein 5 expression in C2C12 cells using Rho GTPase activating protein 5 antibody (Cat#AGI1361, 1:2,000). Green, isotype control; red, Rho GTPase activating protein 5.
    detail
  • ICC - KD-Validated Anti-Rho GTPase activating protein 5 Rabbit Monoclonal Antibody AGI1361
    Immunocytochemical staining of C2C12 cells with Rho GTPase activating protein 5 antibody (Cat#AGI1361, 1:1,000). Nuclei were stained blue with DAPI; Rho GTPase activating protein 5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession Q13017
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 23GB3860
Calculated MW Predicted, 172 kDa , observed, 190 kDa
Gene Name ARHGAP5
Aliases ARHGAP5; Rho GTPase Activating Protein 5; P190-B; Rho-Type GTPase-Activating Protein 5; Rho GTPase-Activating Protein 5; Growth Factor Independent 2; P190BRhoGAP; RhoGAP5; RHOGAP5; GFI2; P100 RasGAP-Associated P105 Protein; P105 RhoGAP; P190BRHOGAP
Immunogen A synthesized peptide derived from human RhoGAP
Additional Information
Gene ID 394
Other Names Rho GTPase-activating protein 5, Rho-type GTPase-activating protein 5, p190-B, ARHGAP5, RHOGAP5
Protein Information
Name ARHGAP5
Synonyms RHOGAP5
Function GTPase-activating protein for Rho family members (PubMed:8537347).
Cellular Location Cytoplasm. Cell membrane; Peripheral membrane protein. Note=Also membrane-associated in a fibrillar pattern that colocalizes with the alpha5-beta1 integrin receptor (ITGA5/ITGB1) for fibronectin.
Tissue Location Detected in skin fibroblasts (at protein level) (PubMed:8537347).
Research Areas
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$ 149.00
$ 499.00
Cat# AGI1361
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