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>   home   >   Products   >   Primary Antibodies   >   Antibody Collections   >   KD-Validated Antibodies   >   KD-Validated Anti-Methylthioadenosine phosphorylase Rabbit Monoclonal Antibody   

KD-Validated Anti-Methylthioadenosine phosphorylase Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-Methylthioadenosine phosphorylase Rabbit Monoclonal Antibody AGI1455
    Western blotting analysis using anti-Methylthioadenosine phosphorylase antibody (Cat#61807). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Methylthioadenosine phosphorylase antibody (Cat#61807, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • WB - KD-Validated Anti-Methylthioadenosine phosphorylase Rabbit Monoclonal Antibody AGI1455
    Western blotting analysis using anti-Methylthioadenosine phosphorylase antibody (Cat#61807). Methylthioadenosine phosphorylase expression in wild type (WT) and Methylthioadenosine phosphorylase shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Methylthioadenosine phosphorylase antibody (Cat#61807, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • FC - KD-Validated Anti-Methylthioadenosine phosphorylase Rabbit Monoclonal Antibody AGI1455
    Flow cytometric analysis of Methylthioadenosine phosphorylase expression in HT-1080 cells using Methylthioadenosine phosphorylase antibody (Cat#61807, 1:2,000). Green, isotype control; red, Methylthioadenosine phosphorylase.
    detail
  • ICC - KD-Validated Anti-Methylthioadenosine phosphorylase Rabbit Monoclonal Antibody AGI1455
    Immunocytochemical staining of HT-1080 cells with Methylthioadenosine phosphorylase antibody (Cat#61807, 1:1,000). Nuclei were stained blue with DAPI; Methylthioadenosine phosphorylase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession Q13126
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 23GB4560
Calculated MW Predicted, 31 kDa , observed, 29 kDa
Gene Name MTAP
Aliases MTAP; Methylthioadenosine Phosphorylase; MSAP; S-Methyl-5'-Thioadenosine Phosphorylase; 5'-Methylthioadenosine Phosphorylase; MTA Phosphorylase; MTAPase; C86fus; Epididymis Secretory Sperm Binding Protein; Epididymis Luminal Protein 249; MeSAdo Phosphorylase; EC 2.4.2.28; HEL-249; DMSMFH; C86FUS; DMSFH; LGMBF; BDMF
Immunogen A synthesized peptide derived from human MTAP
Additional Information
Gene ID 4507
Other Names S-methyl-5'-thioadenosine phosphorylase {ECO:0000255|HAMAP-Rule:MF_03155}, 2.4.2.28 {ECO:0000255|HAMAP-Rule:MF_03155}, 5'-methylthioadenosine phosphorylase {ECO:0000255|HAMAP-Rule:MF_03155}, MTA phosphorylase {ECO:0000255|HAMAP-Rule:MF_03155}, MTAP {ECO:0000255|HAMAP-Rule:MF_03155}, MTAPase {ECO:0000255|HAMAP-Rule:MF_03155}, MTAP {ECO:0000255|HAMAP-Rule:MF_03155}, MSAP
Protein Information
Name MTAP {ECO:0000255|HAMAP-Rule:MF_03155}
Synonyms MSAP
Function Catalyzes the reversible phosphorylation of S-methyl-5'- thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S-adenosylmethionine. Has broad substrate specificity with 6-aminopurine nucleosides as preferred substrates.
Cellular Location Cytoplasm. Nucleus {ECO:0000255|HAMAP- Rule:MF_03155}
Tissue Location Ubiquitously expressed.
Research Areas
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$ 149.00
$ 499.00
Cat# AGI1455
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