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KD-Validated Anti-TSFM Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-TSFM Rabbit Monoclonal Antibody AGI1469
    Western blotting analysis using anti-TSFM antibody (Cat#AGI1469). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-TSFM antibody (Cat#AGI1469, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-TSFM Rabbit Monoclonal Antibody AGI1469
    Western blotting analysis using anti-TSFM antibody (Cat#AGI1469). TSFM expression in wild type (WT) and TSFM shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-TSFM antibody (Cat#AGI1469, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • FC - KD-Validated Anti-TSFM Rabbit Monoclonal Antibody AGI1469
    Flow cytometric analysis of TSFM expression in HeLa cells using TSFM antibody (Cat#AGI1469, 1:2,000). Green, isotype control; red, TSFM.
    detail
  • ICC - KD-Validated Anti-TSFM Rabbit Monoclonal Antibody AGI1469
    Immunocytochemical staining of HeLa cells with TSFM antibody (Cat#AGI1469, 1:1,000). Nuclei were stained blue with DAPI; TSFM was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession P43897
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 23GB4710
Calculated MW Predicted, 35 kDa , observed, 32 kDa
Gene Name TSFM
Aliases TSFM; Ts Translation Elongation Factor, Mitochondrial; Elongation Factor Ts, Mitochondrial; EF-TS; Mitochondrial Elongation Factor Ts; EF-Tsmt; EF-TSMT; EF-TsMt; EFTSMT; EF-Ts; EFTS
Immunogen A synthesized peptide derived from human TSFM
Additional Information
Gene ID 10102
Other Names Elongation factor Ts, mitochondrial {ECO:0000255|HAMAP-Rule:MF_03135}, EF-Ts {ECO:0000255|HAMAP-Rule:MF_03135}, EF-TsMt {ECO:0000255|HAMAP-Rule:MF_03135}, TSFM {ECO:0000255|HAMAP-Rule:MF_03135}
Protein Information
Name TSFM {ECO:0000255|HAMAP-Rule:MF_03135}
Function Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF- Tu.GTP complex up to the GTP hydrolysis stage on the ribosome.
Cellular Location Mitochondrion.
Tissue Location Expressed in all tissues, with the highest levels of expression in skeletal muscle, liver and kidney
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI1469
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