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KD-Validated Anti-Eukaryotic translation elongation factor 2 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-Eukaryotic translation elongation factor 2 Rabbit Monoclonal Antibody AGI1573
    Western blotting analysis using anti-Eukaryotic translation elongation factor 2 antibody (Cat#AGI1573). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Eukaryotic translation elongation factor 2 antibody (Cat#AGI1573, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • WB - KD-Validated Anti-Eukaryotic translation elongation factor 2 Rabbit Monoclonal Antibody AGI1573
    Western blotting analysis using anti-Eukaryotic translation elongation factor 2 antibody (Cat#AGI1573). Eukaryotic translation elongation factor 2 expression in wild type (WT) and Eukaryotic translation elongation factor 2 shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Eukaryotic translation elongation factor 2 antibody (Cat#AGI1573, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
    detail
  • FC - KD-Validated Anti-Eukaryotic translation elongation factor 2 Rabbit Monoclonal Antibody AGI1573
    Flow cytometric analysis of Eukaryotic translation elongation factor 2 expression in HepG2 cells using Eukaryotic translation elongation factor 2 antibody (Cat#AGI1573, 1:2,000). Green, isotype control; red, Eukaryotic translation elongation factor 2.
    detail
  • ICC - KD-Validated Anti-Eukaryotic translation elongation factor 2 Rabbit Monoclonal Antibody AGI1573
    Immunocytochemical staining of HepG2 cells with Eukaryotic translation elongation factor 2 antibody (Cat#AGI1573, 1:1,000). Nuclei were stained blue with DAPI; Eukaryotic translation elongation factor 2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession P13639
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 23GB1550
Calculated MW Predicted, 95 kDa , observed, 95 kDa
Gene Name EEF2
Aliases EEF2; Eukaryotic Translation Elongation Factor 2; EEF-2; EF2; Polypeptidyl-TRNA Translocase; Elongation Factor 2; EF-2; Epididymis Secretory Sperm Binding Protein; EC 3.6.5.-; SCA26
Immunogen A synthesized peptide derived from human EEF2
Additional Information
Gene ID 1938
Other Names Elongation factor 2, EF-2, 3.6.5.-, EEF2, EF2
Protein Information
Name EEF2
Synonyms EF2
Function Catalyzes the GTP-dependent ribosomal translocation step during translation elongation (PubMed:26593721). During this step, the ribosome changes from the pre-translocational (PRE) to the post- translocational (POST) state as the newly formed A-site-bound peptidyl- tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively (PubMed:26593721). Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome (PubMed:26593721).
Cellular Location Cytoplasm. Nucleus. Note=Phosphorylation by CSK promotes cleavage and SUMOylation-dependent nuclear translocation of the C- terminal cleavage product.
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI1573
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