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KD-Validated Anti-GATAD2A Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-GATAD2A Rabbit Monoclonal Antibody AGI1580
    Western blotting analysis using anti-GATAD2A antibody (Cat#62251). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-GATAD2A antibody (Cat#62251, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • WB - KD-Validated Anti-GATAD2A Rabbit Monoclonal Antibody AGI1580
    Western blotting analysis using anti-GATAD2A antibody (Cat#62251). GATAD2A expression in wild type (WT) and GATAD2A shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-GATAD2A antibody (Cat#62251, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • FC - KD-Validated Anti-GATAD2A Rabbit Monoclonal Antibody AGI1580
    Flow cytometric analysis of GATAD2A expression in HeLa cells using GATAD2A antibody (Cat#62251, 1:2,000). Green, isotype control; red, GATAD2A.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC
Primary Accession Q86YP4
Reactivity Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 23GB5685
Calculated MW Predicted, 68 kDa , observed , 68 kDa
Gene Name GATAD2A
Aliases GATAD2A; GATA Zinc Finger Domain Containing 2A; GATA Zinc Finger Domain-Containing Protein 2A; Transcriptional Repressor P66-Alpha; P66alpha; P66 Alpha; Hp66alpha; P66ALPHA
Immunogen A synthesized peptide derived from human p66 alpha
Additional Information
Gene ID 54815
Other Names Transcriptional repressor p66-alpha, Hp66alpha, GATA zinc finger domain-containing protein 2A, GATAD2A
Protein Information
Name GATAD2A
Function Transcriptional repressor (PubMed:12183469, PubMed:16415179). Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin (PubMed:16428440, PubMed:28977666). Enhances MBD2-mediated repression (PubMed:12183469, PubMed:16415179). Efficient repression requires the presence of GATAD2B (PubMed:16415179).
Cellular Location Nucleus speckle. Nucleus. Chromosome. Note=Speckled nuclear localization requires both CR1 and CR2 regions (PubMed:16415179). Localizes to sites of DNA damage in a manner partially dependent on ZMYND8 (PubMed:27732854).
Tissue Location Ubiquitous, both in fetal and adult tissues.
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI1580
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